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Difference between revisions of "DTPA"

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{{MitoPedia
{{MitoPedia
|abbr=DTPA
|abbr=DTPA
|description='''DTPA''' (Diethylentriamin-N,N,N',N,N-pentaessigsäure, "Triplex V") is is a chelator (heavy metals) for fluorescence, not cell membrane permeable, use TPEN as cell membrane permeable chelator.  
|description='''DTPA''' (Diethylentriamin-N,N,N',N,N-penta acetic acid, "Triplex V") is a chelator of transition metals, not cell membrane permeable.  
}}
}}
{{MitoPedia methods}}
 
{{MitoPedia topics}}
== Description ==
 
::::* CAS: 67-43-6
::::* Source: EHERE13095000 VWR 0.25 g
::::* M: 393,35 g/mol
::::* Stability constants not included in NIST version of Martell 1989, constants from [http://www.dojindo.com/Images/Product%20Photo/Chelate_Table_of_Stability_Constants.pdf]: Ca2+: 10.74 (EDTA 10.96), Mg2+: 9.3 (EDTA: 8.69) Fe II 16.5 (EDTA 14.3), Fe III 28.6 (EDTA 25.1) Mn II 15.6 (EDTA 14.04), ZN 18.75 (EDTA 16.5), general chelates multivalent ions stronger than EDTA; pKa1 = 2.14, pKa2 = 2.38, pKa3 = 4.26 pKa4 = 8.60, pKa5 = 10.53
::::* Solubility in H2O: ca 4,8 g·l−1 bei 25 °C = 12 mM
 
== DTPA in O2k-FluoRespirometry ==
::::* Final concentration in O2k-chamber: 15 µM
::::* Stock solution:

Revision as of 11:11, 22 August 2017


high-resolution terminology - matching measurements at high-resolution


DTPA

Description

DTPA (Diethylentriamin-N,N,N',N,N-penta acetic acid, "Triplex V") is a chelator of transition metals, not cell membrane permeable.

Abbreviation: DTPA


Description

  • CAS: 67-43-6
  • Source: EHERE13095000 VWR 0.25 g
  • M: 393,35 g/mol
  • Stability constants not included in NIST version of Martell 1989, constants from [1]: Ca2+: 10.74 (EDTA 10.96), Mg2+: 9.3 (EDTA: 8.69) Fe II 16.5 (EDTA 14.3), Fe III 28.6 (EDTA 25.1) Mn II 15.6 (EDTA 14.04), ZN 18.75 (EDTA 16.5), general chelates multivalent ions stronger than EDTA; pKa1 = 2.14, pKa2 = 2.38, pKa3 = 4.26 pKa4 = 8.60, pKa5 = 10.53
  • Solubility in H2O: ca 4,8 g·l−1 bei 25 °C = 12 mM

DTPA in O2k-FluoRespirometry

  • Final concentration in O2k-chamber: 15 µM
  • Stock solution: