Difference between revisions of "DTPA"
From Bioblast
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{{MitoPedia | {{MitoPedia | ||
|abbr=DTPA | |abbr=DTPA | ||
|description='''DTPA''' (Diethylentriamin-N,N,N',N,N-pentaacetic acid, pentetic acid) is a chelator of transition metals, not cell membrane permeable. general chelates multivalent ions stronger than EDTA | |description='''DTPA''' (Diethylentriamin-N,N,N',N,N-pentaacetic acid, pentetic acid,[[(Carboxymethyl)imino]bis(ethylenenitrilo)]-tetra-acetic acid) is a polyaminopolycarboxylic acid (like EDTA) chelator of transition metals, which as a form of calcium or zinc trisodium salt acts by exchanging calcium or zinc ions for a metal with a higher binding capacity. The compound is not cell membrane permeable. In general it chelates multivalent ions stronger than EDTA. The pKa1 = 2.14, pKa2 = 2.38, pKa3 = 4.26 pKa4 = 8.60, pKa5 = 10.53 | ||
}} | }} | ||
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::::* '''Source:''' EHRENSTORFER GMBH (EHERE13095000) or Sigma Aldrich (D-1133). | ::::* '''Source:''' EHRENSTORFER GMBH (EHERE13095000) or Sigma Aldrich (D-1133). | ||
::::* '''FW:''' 393.35 | ::::* '''FW:''' 393.35 | ||
::::* ''' | ::::* '''Chemical formula:''' C<sub>2</sub>H<sub>2</sub>O<sub>2</sub> | ||
::::* '''Solubility:''' It is soluble in water (4,8 g/l at 25 °C), but not readily soluble in ethanol or apolar solvents. | |||
::::* '''Stability constant:''' see: [http://www.dojindo.com/Images/Product%20Photo/Chelate_Table_of_Stability_Constants.pdf]: | ::::* '''Stability constant:''' see: [http://www.dojindo.com/Images/Product%20Photo/Chelate_Table_of_Stability_Constants.pdf]: | ||
:::::{| class="wikitable" | :::::{| class="wikitable" | ||
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== DTPA in | == DTPA in HRFR == | ||
::::* Final concentration in O2k-chamber: 15 µM | '''Fluorimetry:''' | ||
::::* Stock solution: | ::::* '''Final concentration in O2k-chamber:''' 15 µM | ||
::::* '''Stock solution:''' 5 mM | |||
::::* '''Preparation of the 5 mM stock solution of DTPA:''' |
Revision as of 14:14, 22 August 2017
{{MitoPedia |abbr=DTPA |description=DTPA (Diethylentriamin-N,N,N',N,N-pentaacetic acid, pentetic acid,[[(Carboxymethyl)imino]bis(ethylenenitrilo)]-tetra-acetic acid) is a polyaminopolycarboxylic acid (like EDTA) chelator of transition metals, which as a form of calcium or zinc trisodium salt acts by exchanging calcium or zinc ions for a metal with a higher binding capacity. The compound is not cell membrane permeable. In general it chelates multivalent ions stronger than EDTA. The pKa1 = 2.14, pKa2 = 2.38, pKa3 = 4.26 pKa4 = 8.60, pKa5 = 10.53 }}
Description
- CAS: 67-43-6
- Source: EHRENSTORFER GMBH (EHERE13095000) or Sigma Aldrich (D-1133).
- FW: 393.35
- Chemical formula: C2H2O2
- Solubility: It is soluble in water (4,8 g/l at 25 °C), but not readily soluble in ethanol or apolar solvents.
- Stability constant: see: [1]:
Metal DTPA EDTA Ca(II) 10.74 10.96 Mg(II) 9.3 8.69 Fe(II) 16.5 14.3 Fe(III) 28.6 25.1 Mn(II) 15.6 14.04 Zn (II) 18.75 16.5
DTPA in HRFR
Fluorimetry:
- Final concentration in O2k-chamber: 15 µM
- Stock solution: 5 mM
- Preparation of the 5 mM stock solution of DTPA: