Difference between revisions of "Dejean 2002 Biochem Biophys Res Comm"
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{{Publication | {{Publication | ||
|title=Dejean L, Beauvoit B, Bunoust O, Guerin B, Rigoulet M (2002) Activation of Ras cascade increases the mitochondrial enzyme content of respiratory competent yeast. Biochem | |title=Dejean L, Beauvoit B, Bunoust O, Guerin B, Rigoulet M (2002) Activation of Ras cascade increases the mitochondrial enzyme content of respiratory competent yeast. Biochem Biophys Res Comm 293:1383-8. | ||
|authors=Dejean L, Beauvoit B, Bunoust O, Guerin B, Rigoulet M | |info=[http://www.ncbi.nlm.nih.gov/pubmed/12054668 PMID: 12054668] | ||
|authors=Dejean L, Beauvoit B, Bunoust O, Guerin B, Rigoulet M | |||
|year=2002 | |year=2002 | ||
|journal=Biochem | |journal=Biochem Biophys Res Commun | ||
|abstract=We investigated the effects of genetic and physiological modulations of the cAMP–protein kinase A pathway on mitochondrial biogenesis of yeast cells grown on lactate. Yeast mutants with over-activated Ras/adenylate cyclase pathway (i.e., | |abstract=We investigated the effects of genetic and physiological modulations of the cAMP–protein kinase A pathway on mitochondrial biogenesis of yeast cells grown on lactate. Yeast mutants with over-activated Ras/adenylate cyclase pathway (i.e., ''Ras2<sup>val19</sup>, ira1Δira2Δ'') or with a constitutive downstream activation of protein kinases A (i.e., bcyΔ) showed an increase in the mitochondrial enzyme content. In contrast, loss of Ras activity (i.e., ''Ras2'' mutant) resulted in a slight decrease. The treatment by cAMP of a responsive mutant increased the oxidative phosphorylation capacity of cells and increased the transcript level of nuclear genes encoding for mitochondrial proteins. In contrast, the transcript level of mitochondrial DNA genes was unchanged. It is concluded that the Ras/cAMP/protein kinase A pathway is part of the regulatory circuit controlling biogenesis of the oxidative phosphorylation complexes in yeast cells. | ||
|keywords=cAMP, Saccharomyces cerevisiae, Ras mutants, Mitochondria, Cytochromes, Respiration | |keywords=cAMP, ''Saccharomyces cerevisiae'', Ras mutants, Mitochondria, Cytochromes, Respiration | ||
| | |mipnetlab=FR Pessac Diolez P, FR Bordeaux Devin A | ||
|discipline=Mitochondrial Physiology | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration, Genetic knockout;overexpression | |||
|organism=Saccharomyces cerevisiae, Fungi | |||
|preparations=Intact cells | |||
|couplingstates=OXPHOS | |||
|instruments=Oxygraph-2k | |||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
}} | }} | ||
Latest revision as of 12:43, 16 February 2018
| Dejean L, Beauvoit B, Bunoust O, Guerin B, Rigoulet M (2002) Activation of Ras cascade increases the mitochondrial enzyme content of respiratory competent yeast. Biochem Biophys Res Comm 293:1383-8. |
Dejean L, Beauvoit B, Bunoust O, Guerin B, Rigoulet M (2002) Biochem Biophys Res Commun
Abstract: We investigated the effects of genetic and physiological modulations of the cAMP–protein kinase A pathway on mitochondrial biogenesis of yeast cells grown on lactate. Yeast mutants with over-activated Ras/adenylate cyclase pathway (i.e., Ras2val19, ira1Δira2Δ) or with a constitutive downstream activation of protein kinases A (i.e., bcyΔ) showed an increase in the mitochondrial enzyme content. In contrast, loss of Ras activity (i.e., Ras2 mutant) resulted in a slight decrease. The treatment by cAMP of a responsive mutant increased the oxidative phosphorylation capacity of cells and increased the transcript level of nuclear genes encoding for mitochondrial proteins. In contrast, the transcript level of mitochondrial DNA genes was unchanged. It is concluded that the Ras/cAMP/protein kinase A pathway is part of the regulatory circuit controlling biogenesis of the oxidative phosphorylation complexes in yeast cells. • Keywords: cAMP, Saccharomyces cerevisiae, Ras mutants, Mitochondria, Cytochromes, Respiration
• O2k-Network Lab: FR Pessac Diolez P, FR Bordeaux Devin A
Labels: MiParea: Respiration, Genetic knockout;overexpression
Organism: Saccharomyces cerevisiae, Fungi
Preparation: Intact cells
Coupling state: OXPHOS
HRR: Oxygraph-2k
