Dela MiP2010
| Kraunsoe R, Boushel R, Hansen CN, Schjerling P, Qvortrup K, Stocke M, Mikines KJ, Dela F (2010) Mitochondrial respiration in subcutaneous and visceral adipose tissue from patients with morbid obesity. Mitochondr. Physiol. Network 15.6: 50-51. |
Kraunsoe R, Boushel R, Hansen CN, Schjerling P, Qvortrup K, Stocke M, Mikines KJ, Dela F (2010) Mitochondr. Physiol. Network
Abstract: Adipose tissue exerts important endocrine and metabolic functions in health and disease. Yet the bioenergetics of this tissue is not characterized in humans and possible regional differences are not elucidated. Using high-resolution respirometry, mitochondrial respiration was quantified in human abdominal subcutaneous and intra-abdominal, visceral (omentum majus) adipose tissue from biopsies obtained in twenty obese patients undergoing bariatric surgery. mtDNA and gDNA were determined by PCR technique for estimation of mitochondrial density. Adipose tissue samples were permeabilized and respirometric measurements were performed in duplicate at 37 °C. Substrates [glutamate(G) + malate(M) + octanoyl carnitine(Oct) + succinate(S)] were added sequentially to provide electrons to Complexes CI + CII. ADP (D) for State 3 respiration was added after GM. Non-coupled respiration was measured after addition of FCCP. Visceral fat contained more mitochondria per mg tissue than subcutaneous fat, but the cells were smaller. Robust, stable oxygen fluxes were found in both tissues, and coupled State 3 (GMOctSD) and non-coupled respiration were significantly (P<0.05) higher in visceral (0.95±0.05 and 1.15±0.06 pmol O2∙s-1∙mg-1, respectively) compared with subcutaneous (0.76±0.04 and 0.98±0.05 pmol O2∙s-1∙mg-1, respectively) adipose tissue. Expressed per mtDNA, visceral adipose tissue had significantly (P<0.05) lower mitochondrial respiration. Substrate control ratios were higher and uncoupling control ratio lower (P<0.05) in visceral compared with subcutaneous adipose tissue.
Visceral fat is bioenergetically more active and more sensitive to mitochondrial substrate supply than subcutaneous fat. OXPHOS has a higher relative activity in visceral compared with subcutaneous adipose tissue.
• O2k-Network Lab: DK_Copenhagen_ DelaF
Labels:
Organism: Human
Preparation: Intact Cell; Cultured; Primary"Intact Cell; Cultured; Primary" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Coupling; Membrane Potential"Coupling; Membrane Potential" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Substrate; Glucose; TCA Cycle"Substrate; Glucose; TCA Cycle" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Fatty Acid"Fatty Acid" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Oxygraph-2k
Adipose tissue, mtDNA
