Difference between revisions of "Diaz-Casado 2014 Abstract SECF"
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|title=Melatonin restores mitochondrial regulatory gene expression and function | |title=Melatonin restores mitochondrial regulatory gene expression and function | ||
in the zebrafish model of parkinsonism. | in the zebrafish model of parkinsonism. | ||
|authors=DĂaz-Casado E, Lima-Cabello E, Doerrier C, GarcĂa JA, Escames G, Acuña-Castroviejo D | |authors=DĂaz-Casado E, Lima-Cabello E, Doerrier C, GarcĂa JA, Escames G, Acuña-Castroviejo D | ||
|year=2014 | |year=2014 | ||
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mechanisms are directly related to mitochondrial dysfunction and bioenergetic failure, which promotes neuronal death. The neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), which specifically inhibits mitochondrial complex I activity, is usually used to yied parkinsonism in roedents and zebrafish. The association between mitochondrial dysfunction and neurodegeneration is gaining experimental support in PD, which can be | mechanisms are directly related to mitochondrial dysfunction and bioenergetic failure, which promotes neuronal death. The neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), which specifically inhibits mitochondrial complex I activity, is usually used to yied parkinsonism in roedents and zebrafish. The association between mitochondrial dysfunction and neurodegeneration is gaining experimental support in PD, which can be | ||
associated with mutations in mitochondria-associated genes including DJ-1, alpha-synuclein, Park-2 and PINK-1. Because mitochondria are the main target of melatonin actions, we evaluated here whether its neuroprotective actions depend on the regulation of these genes in the zebrafish model of PD. | associated with mutations in mitochondria-associated genes including DJ-1, alpha-synuclein, Park-2 and PINK-1. Because mitochondria are the main target of melatonin actions, we evaluated here whether its neuroprotective actions depend on the regulation of these genes in the zebrafish model of PD. | ||
To generate a zebrafish model of PD with the use of MPTP, looking for molecular targets involved in the neuroprotective effects of melatonin. | |||
Zebrafish embryos of the AB line were used at 24 hpf. 70 zebrafish embryos were randomly divided in three groups; Control Group (C); MPTP Group (MPTP), treated with MPTP (600 ÎŒM), and aMT Group (aMT), treated with MPTP (600 ÎŒM) and aMT (1ÎŒM) during 48h. Mitochondrial respiratory function and analysis of gene | |||
expression (qRT-PCR and Western Blot) of THase, Park-7, alpha-synuclein, Park-2, Park-6, and iNOS isoform were performed. | |||
|mipnetlab=[[ES Granada Acuna-Castroviejo D]] | |||
}} | }} | ||
{{Labeling}} | {{Labeling}} | ||
Revision as of 10:32, 30 September 2015
| Melatonin restores mitochondrial regulatory gene expression and function
in the zebrafish model of parkinsonism. |
Link:
DĂaz-Casado E, Lima-Cabello E, Doerrier C, GarcĂa JA, Escames G, Acuña-Castroviejo D (2014)
Event: SECF
Parkinsonâs disease (PD) is a neurological disorder characterized by the progressive loss of dopaminergic (DA) neurons. Oxidative stress and inflammation are two key processes in the pathophysiology of PD. Both mechanisms are directly related to mitochondrial dysfunction and bioenergetic failure, which promotes neuronal death. The neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), which specifically inhibits mitochondrial complex I activity, is usually used to yied parkinsonism in roedents and zebrafish. The association between mitochondrial dysfunction and neurodegeneration is gaining experimental support in PD, which can be associated with mutations in mitochondria-associated genes including DJ-1, alpha-synuclein, Park-2 and PINK-1. Because mitochondria are the main target of melatonin actions, we evaluated here whether its neuroprotective actions depend on the regulation of these genes in the zebrafish model of PD.
To generate a zebrafish model of PD with the use of MPTP, looking for molecular targets involved in the neuroprotective effects of melatonin.
Zebrafish embryos of the AB line were used at 24 hpf. 70 zebrafish embryos were randomly divided in three groups; Control Group (C); MPTP Group (MPTP), treated with MPTP (600 ÎŒM), and aMT Group (aMT), treated with MPTP (600 ÎŒM) and aMT (1ÎŒM) during 48h. Mitochondrial respiratory function and analysis of gene expression (qRT-PCR and Western Blot) of THase, Park-7, alpha-synuclein, Park-2, Park-6, and iNOS isoform were performed.
âą O2k-Network Lab: ES Granada Acuna-Castroviejo D
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