Dlaskova 2010 Biochim Biophys Acta: Difference between revisions
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{{Publication | {{Publication | ||
|title=DlaskovΓ‘ A, Clarke KJ, Porter RK (2010) The role of UCP 1 in production of reactive oxygen species by mitochondria isolated from brown adipose tissue. Biochim Biophys Acta 1797: 1470- | |title=DlaskovΓ‘ A, Clarke KJ, Porter RK (2010) The role of UCP 1 in production of reactive oxygen species by mitochondria isolated from brown adipose tissue. Biochim Biophys Acta 1797:1470-6. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/20416274 PMID: 20416274]; [http://www.tara.tcd.ie/bitstream/2262/40404/1/The%20role%20of%20UCP%201%20in%20production%20of%20reactive%20oxygen%20species%20by%20mitochondria%20isolated%20from%20brown%20adipose%20tissue.pdf PDF] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/20416274 PMID: 20416274]; [http://www.tara.tcd.ie/bitstream/2262/40404/1/The%20role%20of%20UCP%201%20in%20production%20of%20reactive%20oxygen%20species%20by%20mitochondria%20isolated%20from%20brown%20adipose%20tissue.pdf PDF] | ||
|authors=Dlaskova A, Clarke KJ, Porter RK | |authors=Dlaskova A, Clarke KJ, Porter RK | ||
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|organism=Mouse | |organism=Mouse | ||
|tissues=Fat | |tissues=Fat | ||
|preparations=Isolated | |preparations=Isolated mitochondria | ||
|enzymes=Uncoupling protein | |enzymes=Uncoupling protein | ||
|injuries=RONS | |injuries=Oxidative stress;RONS | ||
|couplingstates=OXPHOS | |couplingstates=OXPHOS | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
}} | }} |
Revision as of 14:22, 13 February 2015
DlaskovΓ‘ A, Clarke KJ, Porter RK (2010) The role of UCP 1 in production of reactive oxygen species by mitochondria isolated from brown adipose tissue. Biochim Biophys Acta 1797:1470-6. |
Β» PMID: 20416274; PDF
Dlaskova A, Clarke KJ, Porter RK (2010) Biochim Biophys Acta
Abstract: We provide evidence that ablation or inhibition of, uncoupling protein 1 increases the rate of reactive oxygen containing species production by mitochondria from brown adipose tissue, no matter what electron transport chain substrate is used (succinate, glycerol-3-phosphate or pyruvate/malate). Consistent with these data are our observations that (a) the mitochondrial membrane potential is maximal when uncoupling protein 1 is ablated or inhibited and (b) oxygen consumption rates in mitochondria from uncoupling protein 1 knock-out mice, are significantly lower than those from wild-type mice, but equivalent to those from wild-type mice in the presence of GDP. In summary, we show that uncoupling protein 1 can affect reactive oxygen containing species production by isolated mitochondria from brown adipose tissue. β’ Keywords: mitochondria, reactive oxygen species, brown adipose tissue, uncoupling protein 1
β’ O2k-Network Lab: IE_Dublin_Porter RK
Labels: MiParea: Respiration, Genetic knockout;overexpression
Stress:Oxidative stress;RONS Organism: Mouse Tissue;cell: Fat Preparation: Isolated mitochondria Enzyme: Uncoupling protein
Coupling state: OXPHOS
HRR: Oxygraph-2k