Drahota 2005 Cell Biochem Biophys: Difference between revisions
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{{Publication | {{Publication | ||
|title=Drahota Z, Milerova M, Stieglerova A, Skarka L, Houstek J, Ostadal B (2005) Development of cytochrome-c oxidase in rat heart. Cell Biochem Biophys 43: 87-94. | |title=Drahota Z, Milerova M, Stieglerova A, Skarka L, Houstek J, Ostadal B (2005) Development of cytochrome-c oxidase in rat heart. Cell Biochem Biophys 43:87-94. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/16043886 PMID: 16043886] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/16043886 PMID: 16043886] | ||
|authors=Drahota Z, Milerova M, Stieglerova A, Skarka L, Houstek J, Ostadal B | |authors=Drahota Z, Milerova M, Stieglerova A, Skarka L, Houstek J, Ostadal B | ||
|year=2005 | |year=2005 | ||
|journal=Cell Biochem Biophys | |journal=Cell Biochem Biophys | ||
|abstract=Cytochrome | |abstract=Cytochrome ''c'' oxidase (COX) activity of the rat heart was two- to sevenfold activated when the membrane integrity was disrupted by hypotonic swelling, freezing-thawing, or a detergent, indicating that a large portion of COX capacity in intact mitochondria is not active. The effect of detergent was tested in heart mitochondria isolated from 1-, 5-, 15-, 29-, and 60-d-old rats; activation by detergent was up to 20-fold in 1-d-old animals, whereas in mitochondria from 60-d-old rats it was only 7-fold. Our data indicate that the rat heart exhibits significant developmental changes dependent on downregulation of COX activity in the neonatal period. | ||
|keywords=Rat heart, Postnatal development, Cytochrome-c oxidase, Mitochondria, Detergent | |keywords=Rat heart, Postnatal development, Cytochrome-c oxidase, Mitochondria, Detergent | ||
|mipnetlab=CZ Prague Houstek J | |||
|discipline=Biomedicine | |discipline=Biomedicine | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
| | |area=Respiration | ||
|organism=Rat | |organism=Rat | ||
|tissues=Heart | |tissues=Heart | ||
|preparations=Isolated Mitochondria, Oxidase; Biochemical Oxidation | |preparations=Isolated Mitochondria, Oxidase; Biochemical Oxidation | ||
|enzymes=Complex IV; Cytochrome c Oxidase | |||
|couplingstates=OXPHOS | |couplingstates=OXPHOS | ||
| | |instruments=Oxygraph-2k | ||
|discipline=Biomedicine | |discipline=Biomedicine | ||
}} | }} |
Revision as of 06:03, 15 December 2014
Drahota Z, Milerova M, Stieglerova A, Skarka L, Houstek J, Ostadal B (2005) Development of cytochrome-c oxidase in rat heart. Cell Biochem Biophys 43:87-94. |
Drahota Z, Milerova M, Stieglerova A, Skarka L, Houstek J, Ostadal B (2005) Cell Biochem Biophys
Abstract: Cytochrome c oxidase (COX) activity of the rat heart was two- to sevenfold activated when the membrane integrity was disrupted by hypotonic swelling, freezing-thawing, or a detergent, indicating that a large portion of COX capacity in intact mitochondria is not active. The effect of detergent was tested in heart mitochondria isolated from 1-, 5-, 15-, 29-, and 60-d-old rats; activation by detergent was up to 20-fold in 1-d-old animals, whereas in mitochondria from 60-d-old rats it was only 7-fold. Our data indicate that the rat heart exhibits significant developmental changes dependent on downregulation of COX activity in the neonatal period. โข Keywords: Rat heart, Postnatal development, Cytochrome-c oxidase, Mitochondria, Detergent
โข O2k-Network Lab: CZ Prague Houstek J
Labels: MiParea: Respiration
Organism: Rat
Tissue;cell: Heart
Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property., Oxidase; Biochemical Oxidation"Oxidase; Biochemical Oxidation" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Enzyme: Complex IV; Cytochrome c Oxidase"Complex IV; Cytochrome c Oxidase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property.
Coupling state: OXPHOS
HRR: Oxygraph-2k