Difference between revisions of "Dufour 2013 Appl Environ Microbiol"
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{{Publication | {{Publication | ||
|title=Dufour V, Stahl M, Rosenfeld E, Stintzi A, Baysse C (2013) Insights into the mode of action of benzylisothiocyanate on Campylobacter jejuni. Appl Environ Microbiol | |title=Dufour V, Stahl M, Rosenfeld E, Stintzi A, Baysse C (2013) Insights into the mode of action of benzylisothiocyanate on Campylobacter jejuni. Appl Environ Microbiol 79: 6958-6968 | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/24014524 PMID:24014524] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/24014524 PMID:24014524] | ||
|authors=Dufour V, Stahl M, Rosenfeld E, Stintzi A, Baysse C | |authors=Dufour V, Stahl M, Rosenfeld E, Stintzi A, Baysse C |
Revision as of 13:53, 25 October 2013
Dufour V, Stahl M, Rosenfeld E, Stintzi A, Baysse C (2013) Insights into the mode of action of benzylisothiocyanate on Campylobacter jejuni. Appl Environ Microbiol 79: 6958-6968 |
Dufour V, Stahl M, Rosenfeld E, Stintzi A, Baysse C (2013) Appl Environ Microbiol
Abstract: Campylobacter jejuni is a widespread pathogen responsible for most of the food-borne gastrointestinal diseases in Europe. The use of natural antimicrobial molecules is a promising alternative to antibiotic treatments for pathogen control in the food industry. Isothiocyanates are natural antimicrobial compounds, which also display anti-cancer activity. Several studies described the chemoprotective effect of isothiocyanates on eukaryotic cells, but the antimicrobial mechanism is still poorly understood.We investigated the early cellular response of C. jejuni to benzylisothiocyanate by both transcriptomic and physiological approaches. The transcriptomic response of C. jejuni to benzylisothiocyanate showed upregulation of heat shock response genes and an impact on energy metabolism. The oxygen consumption was progressively impaired by benzylisothiocyanate treatment as revealed by high-resolution respirometry, while the ATP content increased soon after benzylisothiocyanate exposition, which suggests a shift in the energy metabolism balance. Finally, benzylisothiocyanate induced intracellular protein aggregation. These results indicate that benzylisothiocyanate affects C. jejuni by targeting proteins, resulting in the disruption of major metabolic processes and eventually leading to cell death. β’ Keywords: energy metabolism, microarray, gene transcription, isothiocyanates, pathogen
Labels: MiParea: Respiration
Preparation: Intact cells
Regulation: O2"O2" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. Coupling state: ROUTINE
HRR: Oxygraph-2k