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| {{Product
| | #REDIRECT [[O2k-Fluo Smart-Module]] |
| |description='''Fluorescence-Sensor''': LED with specified wavelength, photodiode, [[Filter-Cap]] attached with specific optical filter for the LED and/or photodiode. See [[Fluorescence-Sensor Green]] and [[Fluorescence-Sensor Cyan]].
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| |product id=442##
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| |product type=O2k, MultiSensor, O2k-Fluorescence LED2-Module, O2k-Manual
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| |info=[[O2k-Fluorescence LED2-Module]]
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| |product image=[[File:Fluorescence-Sensor Green.JPG|180px|right|link=]]
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| }}
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| __TOC__
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| == O2k-Manual ==
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| === Select the Fluorescence-Sensors ===
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| Switching between different excitation wavelengths and filters is achieved by simply exchanging the fluorescence sensors. Two types of optical sensors are supplied with different LEDs for fluorescence excitation, and the effective spectra of the LEDs are modified by filters:
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| # [[Fluorescence-Sensor Green|LED 525 nm wavelength: Green]]. The installed Filter-Cap is ready for H2O2 measurement with Amplex® UltraRed.
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| # [[Fluorescence-Sensor Cyan|LED 480 nm wavelength: Cyan]]. The installed Filter-Cap is ready for measurements with Magnesium green® or Calcium green®. A different filter is used for measurement of mt-membrane potential with safranin. | |
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| '''Setup - next step: [[Filter-Cap#Mounting_a_Filter-Cap|Mounting a Filter-Cap]]'''
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| [[File:Connect Fluorescence-Sensor lettered.JPG|400px|right|link=]]
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| === Connect Fluorescence-Sensor to O2k ===
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| For recording of fluorescence signals, insert the black sensor into the window of the O2k chamber as far as possible, aligning the Sensor-Guide Sector with the Sensor-Guide of the O2k-Front Fixation. Then connect the cable of the Fluorescence-Sensor to the Fluorescence-Sensor Plug of the Fluorescence-Control Unit.
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| === Stoppers ===
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| Use only [[Stopper\black PEEK\conical Shaft\central Port|black PEEK stoppers]] in conjunction with fluorometric measurements. If necessary, replace the previous white PVDF stoppers. The black stoppers can be used for all HRR applications in general. See [[MiPNet12.06]] for calibration of the O2k-chamber volume, which is identical for PEEK and PVDF stoppers.
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| '''O2k-Manual: [[O2k-Fluorescence_LED2-Module#Electronic_settings|Electronic settings]]'''
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