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Difference between revisions of "Fragile samples"

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{{Technical service}}


Unfortunately, the rigorous stirring required for high- resolution respirometryΒ  causes problems for organisms / samples that are easily damaged by mechanical force, like fish eggs or beads used to grow adherent cells. Therefore the [[Adherent cells]] in the oxygraph problem is linked to the fragile sample problem.
One possible work around for fragile samples (including large beads)is to construct a cage from wire that holds the actual sample. A few threads of this wire cage can be used to position the cage inside the chamber by holding it against the chamber walls. This requires samples large enough to be retained by a wire cage wide-meshed enough to allow for sufficient stirring of the liquid.
However, this and otherΒ  methods are always a compromise. Experiences with these or other methods would be extremely welcome on this page and on its accompanying Discussion page!
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Latest revision as of 08:12, 9 May 2015