Hagl 2015 J Alzheimers Dis
| Hagl S, Grewal R, Ciobanu I, Helal A, Khayyal MT, Muller WE, Eckert GP (2015) Rice bran extract compensates mitochondrial dysfunction in a cellular model of early Alzheimerβs disease. J Alzheimers Dis 43:927-38. |
Hagl S, Grewal R, Ciobanu I, Helal A, Khayyal MT, Muller WE, Eckert GP (2015) J Alzheimers Dis
Abstract: Mitochondrial dysfunction plays an important role in brain aging and has emerged to be an early event in Alzheimer's disease (AD), contributing to neurodegeneration and the loss of physical abilities seen in patients suffering from this disease. We examined mitochondrial dysfunction in a cell culture model of AD (PC12APPsw cells) releasing very low amyloid-Ξ² (AΞ²40) levels and thus mimicking early AD stages. Our data show that these cells have impaired energy metabolism, low ATP levels, and decreased endogenous mitochondrial respiration. Furthermore, protein levels of PGC1Ξ± as well as of Mitofusin 1 were decreased. PC12APPsw cells also showed an increased mitochondrial content, probably due to an attempt to compensate the impaired mitochondrial function. Recent data showed that stabilized rice bran extract (RBE) protects from mitochondrial dysfunction in vivo [24]. To assess the effect of a RBE on mitochondrial function, we treated PC12APPsw cells for 24 h with RBE. Key components of RBE are oryzanols, tocopherols, and tocotrienols, all substances that have been found to exert beneficial effects on mitochondrial function. RBE incubation elevated ATP production and respiratory rates as well as PGC1Ξ± protein levels in PC12APPsw cells, thus improving the impaired mitochondrial function assessed in our cell culture AD model. Therefore, RBE represents to be a promising nutraceutical for the prevention of AD. β’ Keywords: Alzheimerβs disease, Bioenergetics, Mitochondria, Mitochondrial dynamics, Nitrosative stress, Nutrition, Respiration, Rice bran extract, PC12 rat pheochromocytoma cells
β’ O2k-Network Lab: DE Giessen Eckert GP, DE Frankfurt Eckert GP
Labels: MiParea: Respiration, Patients
Pathology: Alzheimer's
Organism: Rat Tissue;cell: Nervous system, Kidney, Other cell lines Preparation: Permeabilized cells
Coupling state: LEAK, OXPHOS, ET
Pathway: N, S, CIV, NS, ROX
HRR: Oxygraph-2k
