Difference between revisions of "Hoeks 2008 FEBS Lett"
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}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration, mt-Biogenesis; mt-density, Exercise physiology; nutrition; life style | |area=Respiration, mt-Biogenesis;mt-density, Exercise physiology;nutrition;life style | ||
|organism=Rat | |organism=Rat | ||
|tissues=Skeletal muscle | |tissues=Skeletal muscle | ||
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|injuries=RONS; Oxidative Stress | |injuries=RONS; Oxidative Stress | ||
|diseases=Obesity | |diseases=Obesity | ||
|topics=Substrate | |topics=Substrate, Fatty Acid | ||
|couplingstates=OXPHOS | |couplingstates=OXPHOS | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|discipline=Mitochondrial Physiology, Biomedicine | |discipline=Mitochondrial Physiology, Biomedicine | ||
}} | }} | ||
Revision as of 18:13, 11 August 2013
| Hoeks J, Briedรฉ JJ, de Vogel J, Schaart G, Nabben M, Moonen-Kornips E, Hesselink MK, Schrauwen P (2008) Mitochondrial function, content and ROS production in rat skeletal muscle: Effect of high-fat feeding. FEBS Lett 582: 510-516. |
Hoeks J, Briede JJ, de Vogel J, Schaart G, Nabben M, Moonen-Kornips E, Hesselink MK, Schrauwen P (2008) FEBS Lett
Abstract: A high intake of dietary fat has been suggested to diminish mitochondrial functioning in skeletal muscle, possibly attributing to muscular fat accumulation. Here we show however, that an 8-week high-fat dietary intervention did not affect intrinsic functioning of rat skeletal muscle mitochondria assessed by respirometry, neither on a carbohydrate- nor on a lipid-substrate. Interestingly, PPARGC1A protein increased by approximately 2-fold upon high-fat feeding and we observed inconsistent results on different markers of mitochondrial density. Mitochondrial ROS production, assessed by electron spin resonance spectroscopy remained unaffected. Intramyocellular lipid levels increased significantly illustrating that a reduced innate mitochondrial function is not a prerequisite for intra-muscular fat accumulation. โข Keywords: PGC-1ฮฑ
โข O2k-Network Lab: NL_Maastricht_Schrauwen P
Labels: MiParea: Respiration, mt-Biogenesis;mt-density, Exercise physiology;nutrition;life style
Pathology: Obesity
Stress:RONS; Oxidative Stress"RONS; Oxidative Stress" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property.
Organism: Rat
Tissue;cell: Skeletal muscle
Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Regulation: Substrate, Fatty Acid"Fatty Acid" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. Coupling state: OXPHOS
HRR: Oxygraph-2k
