Difference between revisions of "Horgan 1978 Aust J Biol Sci"
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{{Publication | {{Publication | ||
|title=Horgan | |title=Horgan DJ (1978) A spectrophotometric assay of ATP synthesized by sarcoplasmic reticulum. Aust J Biol Sci 31(1):21-4. | ||
|info=[[PMID:98139]] | |info=[[PMID:98139]] | ||
|authors=Horgan Douglas J | |authors=Horgan Douglas J | ||
|year=1978 | |year=1978 | ||
|journal=Aust J Biol Sci | |journal=Aust J Biol Sci | ||
|abstract=The problems encountered with a coupled enzyme assay for ATP using glucose, hexokinase and glucose-6-phosphate dehydrogenase are discussed and a modification where fructose and glucosephosphate isomerase were substituted for glucose is described. This modified assay was used | |abstract=The problems encountered with a coupled enzyme assay for ATP using glucose, hexokinase and glucose-6-phosphate dehydrogenase are discussed and a modification where fructose and glucosephosphate isomerase were substituted for glucose is described. This modified assay was used successfully to measure the ATP synthesized by reversal of the sarcoplasmic reticulum ATPase. ATP synthesized by adenylate kinase contaminating the sarcoplasmic reticulum was easily corrected for by a subtraction procedure. | ||
|editor=[[Cardoso Luiza HD]] | |editor=[[Cardoso Luiza HD]] | ||
}} | }} | ||
== Cited by == | |||
{{Template:Cited by Cardoso 2021 MitoFit MgG}} | |||
{{Labeling | {{Labeling | ||
|additional=MitoFit 2021 MgG | |additional=MitoFit 2021 MgG-PW | ||
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Latest revision as of 11:42, 9 February 2022
| Horgan DJ (1978) A spectrophotometric assay of ATP synthesized by sarcoplasmic reticulum. Aust J Biol Sci 31(1):21-4. |
Horgan Douglas J (1978) Aust J Biol Sci
Abstract: The problems encountered with a coupled enzyme assay for ATP using glucose, hexokinase and glucose-6-phosphate dehydrogenase are discussed and a modification where fructose and glucosephosphate isomerase were substituted for glucose is described. This modified assay was used successfully to measure the ATP synthesized by reversal of the sarcoplasmic reticulum ATPase. ATP synthesized by adenylate kinase contaminating the sarcoplasmic reticulum was easily corrected for by a subtraction procedure.
• Bioblast editor: Cardoso Luiza HD
Cited by
- Cardoso et al (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. Bioenerg Commun 2021.1. doi:10.26124/bec:2021-0001
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MitoFit 2021 MgG-PW
