|
|
| (27 intermediate revisions by 5 users not shown) |
| Line 1: |
Line 1: |
| [[File:O2k-TIP2k.jpg|right|300px|caption]] | | #REDIRECT [[MiPNet16.01 IOC61]] |
| '''O2k-Workshop on High-Resolution Respirometry - Introductory and Advanced'''
| |
| | |
| Schröcken, Vorarlberg, Austria 26 April - 1 May, 2011.
| |
| | |
| Hotel Körbersee
| |
| | |
| For some pictures, see [[IOC60_Photos|IOC60]]
| |
| | |
| More information on IOC61: http://www.oroboros.at/index.php?nextcourse
| |
| | |
| [http://www.oroboros.at/index.php?oroboros-contact '''Contact''']: Katrin Stecher, ''administration''; Email [[Has mailaddress::{{{mailaddress|katrin.stecher@oroboros.at}}}]]
| |
| | |
| | |
| ==Course structure==
| |
| | |
| * Introductory and advanced groups will be working in parallel session, to adjust the programme optimally to the specific needs of the participants in different workpackages.
| |
| | |
| * Hot Topics in Mitochondrial Physiology: 10+5 min
| |
| | |
| ==Abstracts==
| |
| | |
| *[[:IOC61_Abstracts|Look at Abstracts >>]]
| |
| | |
| *[http://www.bioblast.at/index.php/Special:AbstractsExtension?event=IOC61 Add your Abstract >>]
| |
| | |
| For the abstract format, see [[Author_IOC61]].
| |
| | |
| == Combined introductory and advanced O2k-Workshop with parallel sessions ==
| |
| | |
| | |
| ::'''A: Introductory lectures and hands-on experiments for basic HRR in small working teams:'''
| |
| | |
| '''A1. General [[HRR]] topics'''
| |
| * O2k-assembly,
| |
| * [[Polarographic oxygen sensor]] (OroboPOS) service,
| |
| * POS calibration,
| |
| * [[DatLab 4.3|DatLab]] software: principles and operation,
| |
| * [[Titration-Injection-microPump]] - TIP2k.
| |
| '''A2. Protocols in HRR'''
| |
| * Introduction to fundamental elements of mitochondrial respiratory physiology,
| |
| * Design and examples of experimental protocols with intact cells, permeabilized cells and tissues, isolated mitochondria.
| |
| '''A3. Hands-on experiments with the O2k'''
| |
| * Measurement of [[instrumental background oxygen flux]], manual and TIP2k-feedback control,
| |
| * Intact cells: Respirometric reference assay with yeast, [[phosphorylation control protocol]],
| |
| * Preparation of [[permeabilized muscle fibre]]s,
| |
| * HRR with permeabilized muscle fibres.
| |
| '''A4. Presentation and interpretation of [[oxygen flux]]'''
| |
| * Normalization of respiration per tissue or per [[mitochondrial marker]],
| |
| * Coupling control ratios,
| |
| * Substrate control ratios,
| |
| * Generalized flux control ratios.
| |
| '''A5. Overview on O2k-MultiSensor applications - Simultaneous measurement of respiration and:'''
| |
| * Mitochondrial membrane potential (TPP+ electrode),
| |
| * Acidification (pH electrode),
| |
| * Nitric oxide (amperometric),
| |
| * Spectrophotometry (cytochrome spectra),
| |
| * Spectrofluorimetry (Amplex red, safranin, etc.)
| |
| | |
| ::'''B: Advanced users will join in small working teams in parallel sessions, with specific topics:
| |
| '''
| |
| '''B1. O2k-MultiSensor and TIP2k'''
| |
| * Hands-on MultiSensor TPP<sup>+</sup> applications, see [[TPP special interest group during an International Oxygraph Course]]
| |
| * Oxygen kinetics: experimental examples with TIP2k-feedback control (oxystat) and data analysis,
| |
| * ADP kinetics: experimental examples with TIP2-steady state control and data analysis,
| |
| * pH-stat feedback control,
| |
| '''B2. Theoretical perspectives'''
| |
| * Advanced experimental protocols,
| |
| * Quality control - towards [[quality assurance]];
| |
| | |
| * ''Add your topic'':
| |
| | |
| ==Participants and areas of interest==
| |
| | |
| * [[Alexeyev M|'''Alexeyev Mikhail, USA''']]: Mouse modeling of mitochondrial disorders caused by mtDNA mutations.
| |
| * [[Angelin A|'''Angelin Alessia, USA''']]: Function in muscle tissue; in particular human muscle disorder (muscular dystrophy).
| |
| * [[Balboa Castillo E|'''Balboa Castillo Elisa, Chile''']]: Increase in mitochondrial cholesterol and dysfunction in NPC.
| |
| * [[Castelein N|'''Castelein Natascha, Belgium''']]: The role of mitochondria in dietary restriction induced lifespan extension in ''C. elegan''s.
| |
| * [[Gabrielova E|'''Gabrielova Eva, Czech Republic''']]: [[Gabrielova IOC61|Evaluation of oxygen consumption in rat heart mitochondria treatment by polyphenol compounds.]]
| |
| * [[Hals I|'''Hals Ingrid, Norway''']]: Effects of hypoxia in pancreatic beta-cells on mitochondrial functions.
| |
| * [[Hancock S|'''Hancock Saege, USA''']]
| |
| * [[Kasal D|'''Kasal Daniel, Brazil''']]: Modulation of cardiomyocyte respiration by bone marrow stem cells.
| |
| * [[Labieniec M|'''Labieniec Magdalena, Poland''']]: Mitocondrial respiratory capacity; mt membrane potential; calcium homeostasis; mitochondria in health and pathology (diabetes, neurodegenerative diseases, cancer); mt function; ROS
| |
| * [[Le C|'''Le Catherine, USA''']]: Implications of mitochondrial dysfunction in heart disease; cardiomypathy; ROS; mitochondrial function, respiration, membrane potential.
| |
| * [[Magnifico MC|'''Magnifico Maria Chiara, Italy''']]: Measurement of oxygen consumption, respiratory flux and membrane potential in HaCaT cells.
| |
| * [[Mandl M|'''Mandl Markus, Vienna''']]
| |
| * [[Manjeri G|'''Manjeri Ganesh, The Netherlands''']]: Understanding oxygen flux in mitochondrial disorders working with skin fibroblasts.
| |
| * [[Marelsson S|'''Marelsson Sigurdur, Sweden''']]: The role of mitochondrial dysfunction in encephalopaty.
| |
| * [[Morein T|'''Morein Torbjörn, Sweden''']]
| |
| * [[Nuskova H|'''Nuskova Hana, Czech Republic''']]: [[Nuskova IOC61|Mitochondrial Energetics in Health and Disease]].
| |
| * [[Sanz A|'''Sanz Alberto, Finland''']]: Mechanism causing aging and the role of mitochondria in this process.
| |
| * [[Scherer Alves L|'''Scherer Alves Leonardo, USA''']]: Mitochondrial Physiology as a force of human adaptation and evolution to differnet geograpie niches and the relation between the environment and mitochondrial genes in human common diseases.
| |
| * [[Watala C|'''Watala Cezary, Poland''']]: Mitocondrial respiratory capacity; mt membrane potential; calcium homeostasis; mitochondria in health and pathology (diabetes, neurodegenerative diseases, cancer); mt function; ROS
| |
| * [[Yokota T|'''Yokota Takashi, Denmark''']]: The role of mitochondria in chronic heart failure, diabetes, or obesity.
| |
| | |
| == Tutors ==
| |
| | |
| * [[Gnaiger E|'''Erich Gnaiger''']] (Innsbruck Medical University, AT; OROBOROS INSTRUMENTS, AT)
| |
| * [[Fasching M|'''Mario Fasching''']] (OROBOROS INSTRUMENTS, AT)
| |
| * [[Pesta D|'''Dominik Pesta''']] (Innsbruck Medical University, AT)
| |
| * [[Sumbalova Z|'''Zuzana Sumbalova''']] (OROBOROS INSTRUMENTS, AT)
| |
| | |
| ==Discussion==
| |
| | |
| [[Talk:IOC61|Continue the Discussion >>]]
| |
| | |
| [[Category:IOC61]]
| |
| [[Category:OroboroPedia]]
| |
| [[Category:Events]]
| |
