Difference between revisions of "IOC61"
From Bioblast
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* ''Add your topic'': | * ''Add your topic'': | ||
==Participants and areas of interest== | |||
* [[Hals I|'''Ingrid Hals, Norway''']]: To study effects of hypoxia in pancreatic beta-cells on mitochondrial functions. | |||
* [[Sanz A|'''Alberto Sanz, Finland''']]: Mechanism causing aging and the role of mitochondria in this process. | |||
* [[Yokota T|'''Takashi Yokota, Denmark''']]: The role of mitochondria in chronic heart failure, diabetes, or obesity. | |||
== Tutors == | == Tutors == | ||
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* [http://www.oroboros.at/index.php?oroboros-contact '''Contact''']: | * [http://www.oroboros.at/index.php?oroboros-contact '''Contact''']: Katrin Stecher, ''administration''; Email katrin.stecher@oroboros.at | ||
Revision as of 12:58, 11 February 2011
O2k-Workshop on High-Resolution Respirometry - Introductory and Advanced
Schröcken, Vorarlberg, Austria 26 April - 1 May, 2011.
Hotel Körbersee
For some pictures, see IOC60
More information on IOC61: http://www.oroboros.at/index.php?nextcourse
Format
- Introductory and advanced groups will be working in parallel session, to adjust the programme optimally to the specific needs of the participants in different workpackages.
- Hot Topics in Mitochondrial Physiology: 10+5 min
Abstracts
For the abstract format, see Author_IOC61.
Important for adding your abstract: please copy/paste [[Category:IOC61]] into the 'Freetext' field
Combined introductory and advanced O2k-Workshop with parallel sessions
- A: Introductory lectures and hands-on experiments for basic HRR in small working teams:
A1. General HRR topics
- O2k-assembly,
- Polarographic oxygen sensor (OroboPOS) service,
- POS calibration,
- DatLab software: principles and operation,
- Titration-Injection-microPump - TIP2k.
A2. Protocols in HRR
- Introduction to fundamental elements of mitochondrial respiratory physiology,
- Design and examples of experimental protocols with intact cells, permeabilized cells and tissues, isolated mitochondria.
A3. Hands-on experiments with the O2k
- Measurement of instrumental background oxygen flux, manual and TIP2k-feedback control,
- Intact cells: Respirometric reference assay with yeast, phosphorylation control protocol,
- Preparation of permeabilized muscle fibres,
- HRR with permeabilized muscle fibres.
A4. Presentation and interpretation of oxygen flux
- Normalization of respiration per tissue or per mitochondrial marker,
- Coupling control ratios,
- Substrate control ratios,
- Generalized flux control ratios.
A5. Overview on O2k-MultiSensor applications - Simultaneous measurement of respiration and:
- Mitochondrial membrane potential (TPP+ electrode),
- Acidification (pH electrode),
- Nitric oxide (amperometric),
- Spectrophotometry (cytochrome spectra),
- Spectrofluorimetry (Amplex red, safranin, etc.)
- B: Advanced users will join in small working teams in parallel sessions, with specific topics:
B1. O2k-MultiSensor and TIP2k
- Hands-on MultiSensor TPP+ applications,
- Oxygen kinetics: experimental examples with TIP2k-feedback control (oxystat) and data analysis,
- ADP kinetics: experimental examples with TIP2-steady state control and data analysis,
- pH-stat feedback control,
B2. Theoretical perspectives
- Advanced experimental protocols,
- Quality control - towards quality assurance;
- Add your topic:
Participants and areas of interest
- Ingrid Hals, Norway: To study effects of hypoxia in pancreatic beta-cells on mitochondrial functions.
- Alberto Sanz, Finland: Mechanism causing aging and the role of mitochondria in this process.
- Takashi Yokota, Denmark: The role of mitochondria in chronic heart failure, diabetes, or obesity.
Tutors
- Erich Gnaiger (Innsbruck Medical University, AT; OROBOROS INSTRUMENTS, AT)
- Mario Fasching (OROBOROS INSTRUMENTS, AT)
- Dominik Pesta (Innsbruck Medical University, AT)
- Zuzana Sumbalova (OROBOROS INSTRUMENTS, AT)
- Anita Wiethuechter (OROBOROS INSTRUMENTS, AT)
- Contact: Katrin Stecher, administration; Email katrin.stecher@oroboros.at
