Iftikar 2010 JEMBE

Iftikar F, MacDonald JR, Hickey AJ (2010) Thermal limits of portunid crab heart mitochondria: Could more thermo-stable mitochondria advantage invasive species?. JEMBE 395: 232-239.

» doi.org/10.1016/j.jembe.2010.09.005

Iftikar F, MacDonald JR, Hickey AJ (2010) JEMBE

Abstract: The distributions of the native crab Ovalipes catharus and the invasive species Charybdis japonica within New Zealand shores may be defined by thermal tolerance. Doppler ultrasound showed that with increasing temperature O. catharus and C. japonica raised their heart rates (p ≤ 0.05). However, C. japonica showed greater plasticity in heart beat duration, or contraction rate with increasing temperature, while O. catharus was more inclined to increase heart rate, and already had a shorter more rapid contraction at 19 °C. Saponin permeabilized heart fibres enabled tests of mitochondrial function in situ and showed that Leak-I respirational flux was highest for O. catharus at all temperatures. C. japonica showed a greater inner mitochondrial membrane integrity which suggests tighter coupling of oxidative phosphorylation. Although Complex I lost function on exposure to 30 °C in both species, this occurred more rapidly in O. catharus. Complex II function remains stable at high temperature in both species. O. catharus had higher cytochrome c oxidase (CCO) flux at all temperatures compared to C. japonica indicating elevated concentrations of CCO in O. catharus. The scope to increase CCO flux was greater in the more stenothermal O. catharus than in C. japonica. Overall, this study shows substantial differences in heart function and mitochondria between crab species and provides evidence that mitochondrial integrity may limit species survival and potentially future distributions. • Keywords: Complex I failure, Heart rate, Mitochondrial oxidation, Paddle crabs, Q10, crustaceans, crab Ovalipes catharus, Charybdis japonica, cytochrome c oxidase (CCO)

• O2k-Network Lab: NZ Auckland Hickey AJ

Labels:

Stress:z in prep"z in prep" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property.  Organism: Other Non-Mammal"Other Non-Mammal" is not in the list (Human, Pig, Mouse, Rat, Guinea pig, Bovines, Horse, Dog, Rabbit, Cat, ...) of allowed values for the "Mammal and model" property.  Tissue;cell: Cardiac Muscle"Cardiac Muscle" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.  Preparation: Permeabilized Tissue"Permeabilized Tissue" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.  Enzyme: Complex I, Complex II; Succinate Dehydrogenase"Complex II; Succinate Dehydrogenase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property.  Regulation: z in prep"z in prep" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. 

HRR: Oxygraph-2k