Difference between revisions of "Jardim-Messeder 2015 New Phytol"
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{{Publication | {{Publication | ||
|title=Jardim-Messeder D, Caverzan A, Rauber R, de Souza Ferreira E, Margis-Pinheiro M, Galina A (2015) Succinate dehydrogenase (mitochondrial complex II) is a source of reactive oxygen species in plants and regulates development and stress responses. New Phytol 208:776-89. | |title=Jardim-Messeder D, Caverzan A, Rauber R, de Souza Ferreira E, Margis-Pinheiro M, Galina A (2015) Succinate dehydrogenase (mitochondrial complex II) is a source of reactive oxygen species in plants and regulates development and stress responses. New Phytol 208:776-89. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/26082998 PMID: 26082998] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/26082998 PMID: 26082998] | ||
|authors=Jardim-Messeder D, Caverzan A, Rauber R, de Souza Ferreira E, Margis-Pinheiro M, Galina A | |authors=Jardim-Messeder D, Caverzan A, Rauber R, de Souza Ferreira E, Margis-Pinheiro M, Galina A | ||
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|journal=New Phytol | |journal=New Phytol | ||
|abstract=Reactive oxygen species (ROS) are signaling molecules that regulate plant development and responses to stresses. Mitochondria are the source of most ROS in heterotrophic cells, and mitochondrial complex I and complex III are regarded as the main sites of ROS production in plant mitochondria. Recent studies have demonstrated that succinate dehydrogenase (SDH) also contributes to mitochondrial ROS production. However, the ability of SDH to generate ROS in plants is unclear. The aim of this study was to evaluate the role of SDH in mitochondrial ROS production. Our results demonstrated that SDH is a direct source of ROS in ''Arabidopsis thaliana'' and ''Oryza sativa'', and the induction of ROS production by specific SDH inhibitors impaired plant growth. In addition, this effect was accompanied by the down-regulation of cell cycle genes and the up-regulation of stress-related genes. However, the partial inhibition of SDH by a competitive inhibitor decreased ROS production, which was associated with increased shoot and root growth, and prevented the down-regulation of cell cycle genes and the induction of stress-related genes by noncompetitive inhibitors. In conclusion, SDH plays an important role in ROS production, being a direct source of ROS in plant mitochondria and regulating plant development and stress responses. | |abstract=Reactive oxygen species (ROS) are signaling molecules that regulate plant development and responses to stresses. Mitochondria are the source of most ROS in heterotrophic cells, and mitochondrial complex I and complex III are regarded as the main sites of ROS production in plant mitochondria. Recent studies have demonstrated that succinate dehydrogenase (SDH) also contributes to mitochondrial ROS production. However, the ability of SDH to generate ROS in plants is unclear. The aim of this study was to evaluate the role of SDH in mitochondrial ROS production. Our results demonstrated that SDH is a direct source of ROS in ''Arabidopsis thaliana'' and ''Oryza sativa'', and the induction of ROS production by specific SDH inhibitors impaired plant growth. In addition, this effect was accompanied by the down-regulation of cell cycle genes and the up-regulation of stress-related genes. However, the partial inhibition of SDH by a competitive inhibitor decreased ROS production, which was associated with increased shoot and root growth, and prevented the down-regulation of cell cycle genes and the induction of stress-related genes by noncompetitive inhibitors. In conclusion, SDH plays an important role in ROS production, being a direct source of ROS in plant mitochondria and regulating plant development and stress responses. | ||
|keywords=Arabidopsis thaliana ; Oryza sativa, Plant development, Reactive oxygen species (ROS), Duccinate dehydrogenase (SDH) | |keywords=Arabidopsis thaliana ; Oryza sativa, Plant development, Reactive oxygen species (ROS), Duccinate dehydrogenase (SDH), Protoplasts, Amplex Red, Safranin | ||
|mipnetlab=BR Rio de Janeiro Galina A, | |mipnetlab=BR Rio de Janeiro Galina A, | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
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|enzymes=Complex II;succinate dehydrogenase | |enzymes=Complex II;succinate dehydrogenase | ||
|injuries=Oxidative stress;RONS | |injuries=Oxidative stress;RONS | ||
|couplingstates=LEAK, | |couplingstates=LEAK, OXPHOS | ||
|substratestates= | |substratestates=CII | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
}} | }} | ||
Revision as of 15:22, 23 March 2016
| Jardim-Messeder D, Caverzan A, Rauber R, de Souza Ferreira E, Margis-Pinheiro M, Galina A (2015) Succinate dehydrogenase (mitochondrial complex II) is a source of reactive oxygen species in plants and regulates development and stress responses. New Phytol 208:776-89. |
Jardim-Messeder D, Caverzan A, Rauber R, de Souza Ferreira E, Margis-Pinheiro M, Galina A (2015) New Phytol
Abstract: Reactive oxygen species (ROS) are signaling molecules that regulate plant development and responses to stresses. Mitochondria are the source of most ROS in heterotrophic cells, and mitochondrial complex I and complex III are regarded as the main sites of ROS production in plant mitochondria. Recent studies have demonstrated that succinate dehydrogenase (SDH) also contributes to mitochondrial ROS production. However, the ability of SDH to generate ROS in plants is unclear. The aim of this study was to evaluate the role of SDH in mitochondrial ROS production. Our results demonstrated that SDH is a direct source of ROS in Arabidopsis thaliana and Oryza sativa, and the induction of ROS production by specific SDH inhibitors impaired plant growth. In addition, this effect was accompanied by the down-regulation of cell cycle genes and the up-regulation of stress-related genes. However, the partial inhibition of SDH by a competitive inhibitor decreased ROS production, which was associated with increased shoot and root growth, and prevented the down-regulation of cell cycle genes and the induction of stress-related genes by noncompetitive inhibitors. In conclusion, SDH plays an important role in ROS production, being a direct source of ROS in plant mitochondria and regulating plant development and stress responses. • Keywords: Arabidopsis thaliana ; Oryza sativa, Plant development, Reactive oxygen species (ROS), Duccinate dehydrogenase (SDH), Protoplasts, Amplex Red, Safranin
• O2k-Network Lab: BR Rio de Janeiro Galina A
Labels: MiParea: Respiration
Stress:Oxidative stress;RONS
Preparation: Isolated mitochondria
Enzyme: Complex II;succinate dehydrogenase
Coupling state: LEAK, OXPHOS
HRR: Oxygraph-2k
