Kaambre 2013 Front Physiol

Has title::Kaambre T, Chekulayev V, Shevchuk I, Tepp K, Timohhina N, Varikmaa M, Bagur R, Klepinin A, Anmann T, Koit A, Kaldma A, Guzun R, Valvere V, Saks V (2013) Metabolic control analysis of respiration in human cancer tissue. Front Physiol 4:151.

» [[Has info::PMID:23825460 Open Access]]

Was written by::Kaambre T, Was written by::Chekulayev V, Was written by::Shevchuk I, Was written by::Tepp K, Was written by::Timohhina N, Was written by::Varikmaa M, Was written by::Bagur R, Was written by::Klepinin A, Was written by::Anmann T, Was written by::Koit A, Was written by::Kaldma A, Was written by::Guzun R, Was written by::Valvere V, Was written by::Saks V (Was published in year::2013) Was published in journal::Front Physiol

Abstract: [[has abstract::Bioenergetic profiling of cancer cells is of great potential because it can bring forward new and effective therapeutic strategies along with early diagnosis. Metabolic Control Analysis (MCA) is a methodology that enables quantification of the flux control exerted by different enzymatic steps in a metabolic network thus assessing their contribution to the system's function. Our main goal is to demonstrate the applicability of MCA for in situ studies of energy metabolism in human breast and colorectal cancer cells as well as in normal tissues. We seek to determine the metabolic conditions leading to energy flux redirection in cancer cells. A main result obtained is that the adenine nucleotide translocator exhibits the highest control of respiration in human breast cancer thus becoming a prospective therapeutic target. Additionally, we present evidence suggesting the existence of mitochondrial respiratory supercomplexes that may represent a way by which cancer cells avoid apoptosis. The data obtained show that MCA applied in situ can be insightful in cancer cell energetic research.]] • Keywords: has publicationkeywords::Metabolic control analysis, has publicationkeywords::Respiratory chain, has publicationkeywords::Breast and colorectal cancer, has publicationkeywords::Warburg effect, has publicationkeywords::OXPHOS

• O2k-Network Lab: Was published by MiPNetLab::EE Tallinn Saks VA, Was published by MiPNetLab::EE Tallinn Kaambre T

Labels: MiParea: MiP area::Respiration  Pathology: Diseases::Cancer  Stress:Injury and adaptation::Cell death  Organism: Organism::Human  Tissue;cell: tissue and cell::Endothelial;epithelial;mesothelial cell  Preparation: Preparation::Permeabilized tissue  Enzyme: Enzyme::Adenine nucleotide translocase, Enzyme::Complex I, Enzyme::Complex II;succinate dehydrogenase, Enzyme::Complex III, Enzyme::Complex IV;cytochrome c oxidase, Enzyme::Supercomplex  Regulation: Topic::ADP, Topic::Flux control, Topic::Inhibitor, Topic::PCr;Cr 

HRR: Instrument and method::Oxygraph-2k