Difference between revisions of "Knowles 1971 J Biol Chem"
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Revision as of 15:08, 25 November 2015
Knowles AF, Guillory RJ, Racker E (1971) Partial resolution of the enzymes catalyzing oxidative phosphorylation XXIV. A factor required for the binding of mitochondrial adenosine triphosphatase to the inner mitochondrial membrane. J Biol Chem 246:2672-9. |
Β» PMID: 4251853 Open Access; PDF
Knowles AF, Guillory RJ, Racker E (1971) J Biol Chem
Abstract:
- Submitochondrial particles have been sequentially treated with trypsin, urea, and sonic oscillation at an alkaline pH. These TUA particles required addition of a protein (Fc1) in order to render added ATPase (F1) sensitive to dicyclohexylcarbodiimide. Further resolution was obtained by exposure of TUA particles either to 2 M sodium thiocyanate or to 1.5% silicotungstate. These procedures removed a second soluble protein component (Fc2) which was also required for the sensitivity of ATPase to dicyclohexylcarbodiimide.
- Preparations of Fc2 purified from the sodium thiocyanate extract stimulated the 32Pi-ATP exchange reaction and oxidative phosphorylation in silicotungstate-treated submitochondrial particles.
- Treatment of TUA particles with silicotungstate reduced their ability to bind ATPase (F1). Addition of Fc2 restored the ability to bind ATPase. It is therefore proposed that Fc2 is a component which links the mitochondrial ATP-ase to the inner mitochondrial membrane.
β’ Keywords: Oxidative phosphorylation, Enzymes, ATP, Mitochondrial membrane, Submitochondrial particles
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Regulation: ATP production
Made history