Difference between revisions of "Kowaltowski 2014 Abstract MiP2014"
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{{Abstract | {{Abstract | ||
|title=Cardiolipin is a key determinant for mitochondrial DNA stability and segregation. | |title=LuĂ©vano-MartĂnez LA, Forni FM, dos Santos VT, Souza-Pinto NC, Kowaltowski AJ (2014) Cardiolipin is a key determinant for mitochondrial DNA stability and segregation. | ||
|info=[[File:Kowaltowski_AJ.jpg|150px|right|Kowaltowski AJ]] [[Laner 2014 Mitochondr Physiol Network MiP2014|Mitochondr Physiol Network 19.13]] - [http://www.mitophysiology.org/index.php?mip2014 MiP2014] | |info=[[File:Kowaltowski_AJ.jpg|150px|right|Kowaltowski AJ]] [[Laner 2014 Mitochondr Physiol Network MiP2014|Mitochondr Physiol Network 19.13]] - [http://www.mitophysiology.org/index.php?mip2014 MiP2014] | ||
|authors= | |authors=Luevano-Martinez LA, Forni FM, dos Santos VT, Souza-Pinto NC, Kowaltowski AJ | ||
|year=2014 | |year=2014 | ||
|event=MiP2014 | |event=MiP2014 | ||
Line 17: | Line 17: | ||
|area=Respiration, mt-Membrane, mtDNA;mt-genetics | |area=Respiration, mt-Membrane, mtDNA;mt-genetics | ||
|organism=Saccharomyces cerevisiae | |organism=Saccharomyces cerevisiae | ||
|injuries= | |injuries=Hypothermia;hyperthermia | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|event=A1, Oral | |event=A1, Oral |
Revision as of 15:12, 18 May 2015
LuĂ©vano-MartĂnez LA, Forni FM, dos Santos VT, Souza-Pinto NC, Kowaltowski AJ (2014) Cardiolipin is a key determinant for mitochondrial DNA stability and segregation. |
Link:
Mitochondr Physiol Network 19.13 - MiP2014
Luevano-Martinez LA, Forni FM, dos Santos VT, Souza-Pinto NC, Kowaltowski AJ (2014)
Event: MiP2014
Mitochondria play a key role in adapting to stress. Cardiolipin, the main anionic phospholipid in mitochondrial (mt) membranes, is expected to be a determinant in this adaptive mechanism, since it modulates the activity of most membrane proteins.
Here, we used Saccharomyces cerevisiae, subjected to conditions that affect mitochondrial metabolism, as a model to determine the possible role of cardiolipin in stress adaptation. Interestingly, we found that thermal stress promotes an increase in cardiolipin content, modifying both surface charge and the physical state of mt-membranes. These changes have effects on mtDNA segregation and mt-morphology, thus adapting cells to thermal stress. Conversely, since a cardiolipin synthase-null mutant strain is unable to adapt to thermal stress, this effect is cardiolipin-dependent. Interestingly, we found that the loss of cardiolipin specifically affects the segregation of mtDNA to daughter cells, leading to a respiratory deficient phenotype after replication. Furthermore, we provide evidence for a physical interaction between cardiolipin and the mitochondrial nucleoid.
In summary, our results demonstrate that the mitochondrial lipid cardiolipin is a key determinant in the maintenance of mtDNA stability, morphology and segregation.
Funded by: FAPESP, CNPq and NAP-Redoxoma.
âą O2k-Network Lab: BR Sao Paulo Kowaltowski AJ
Labels: MiParea: Respiration, mt-Membrane, mtDNA;mt-genetics
Stress:Hypothermia;hyperthermia"Hypothermia;hyperthermia" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property. Organism: Saccharomyces cerevisiae
HRR: Oxygraph-2k
Event: A1, Oral
MiP2014
Affiliation
Dep BioquĂmica, Inst QuĂmica, Univ SĂŁo Paulo, Brazil. - alicia@iq.usp.br