Kuznetsov 2008 Nat Protoc: Difference between revisions

Revision as of 18:45, 25 February 2011

Kuznetsov AV, Veksler V, Gellerich FN, Saks V, Margreiter R, Kunz WS (2008) Analysis of mitochondrial function in situ in permeabilized muscle fibers, tissues and cells. Nat. Protoc. 3: 965-976.

Kuznetsov AV, Veksler V, Gellerich FN, Saks V, Margreiter R, Kunz WS (2008) Nat. Protoc.

Abstract:

• O2k-Network Lab: EE_Tallinn_SaksV, FR_Grenoble_SaksV

Labels:

HRR: Oxygraph-2k

Corrections required

Table 1: Respiration of rat liver homogenate, 30 °C is actually mechanically permeabilized pig liver measured at 37 °C (Kuznetsov_2002_AnalytBiochem).

The protocol for permeabilized fibres lacks consideration on oxygen limitation encountered when incubations are performed at or below air saturation (Kuznetsov_1998_BTK; Gnaiger_2003_AdvExpMedBiol). Protocols are restricted to simple substrate supply (separate CI- or CII-electron entry into the ETS), whereas full OXPHOS capacity can be obtained only with physiological CI+II substrate combinations (Pesta_2011_Protocols).

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 ==Corrections required==
-In Table 1, respiration of rat liver homogenate at 30 °C is actually mechanically permeabilized pig liver measured at 37 °C ([[Kuznetsov_2002_AnalytBiochem]]).
+::Table 1: ''Respiration of rat liver homogenate, 30 °C'' is actually mechanically permeabilized pig liver measured at 37 °C ([[Kuznetsov_2002_AnalytBiochem]]).
-The protocol for permeabilized fibres lacks consideration on oxygen limitation ([[Kuznetsov_1998_BTK]]). Protocols are restricted to simple substrate supply (separate CI- or CII-electron entry into the ETS), whereas full OXPHOS capacity can be obtained only with physiological CI+II substrate combinations  ([[Pesta_2010_Protocols]]).
+The protocol for permeabilized fibres lacks consideration on oxygen limitation encountered when incubations are performed at or below air saturation ([[Kuznetsov_1998_BTK]]; [[Gnaiger_2003_AdvExpMedBiol]]). Protocols are restricted to simple substrate supply (separate CI- or CII-electron entry into the ETS), whereas full OXPHOS capacity can be obtained only with physiological CI+II substrate combinations ([[Pesta_2011_Protocols]]).