Kuznetsov 2008 Nat Protoc
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| Kuznetsov AV, Veksler V, Gellerich FN, Saks V, Margreiter R, Kunz WS (2008) Analysis of mitochondrial function in situ in permeabilized muscle fibers, tissues and cells. Nat. Protoc. 3: 965-976. |
Kuznetsov AV, Veksler V, Gellerich FN, Saks V, Margreiter R, Kunz WS (2008) Nat. Protoc.
Abstract:
• O2k-Network Lab: EE_Tallinn_SaksV, FR_Grenoble_SaksV
Labels:
HRR: Oxygraph-2k
Corrections required
- Table 1: Respiration of rat liver homogenate, 30 °C is actually mechanically permeabilized pig liver measured at 37 °C (Kuznetsov_2002_AnalytBiochem).
The protocol for permeabilized fibres lacks consideration on oxygen limitation encountered when incubations are performed at or below air saturation (Kuznetsov_1998_BTK; Gnaiger_2003_AdvExpMedBiol). Protocols are restricted to simple substrate supply (separate CI- or CII-electron entry into the ETS), whereas full OXPHOS capacity can be obtained only with physiological CI+II substrate combinations (Pesta_2011_Protocols).
