Difference between revisions of "Lardy 1952 J Biol Chem"
Harrison DK (talk | contribs) |
Harrison DK (talk | contribs) |
||
| Line 19: | Line 19: | ||
The initial rate of oxidation of caprylate to acetoacetate is enhanced by ~P acceptors. This oxidation resulted in P: 0 ratios of 1.1 to 1.4 with creatine as the ~P acceptor and of 1.7 to 2 with glucose as the ~P acceptor. | The initial rate of oxidation of caprylate to acetoacetate is enhanced by ~P acceptors. This oxidation resulted in P: 0 ratios of 1.1 to 1.4 with creatine as the ~P acceptor and of 1.7 to 2 with glucose as the ~P acceptor. | ||
|keywords=oxidative phosphorylation, phosphate acceptor, 2,4-Dinitrophenol | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|organism=Rat | |||
|tissues=Hepatocyte; Liver | |||
|preparations=Isolated Mitochondria | |||
|topics=Respiration; OXPHOS; ETS Capacity | |||
|additional=Made history | |additional=Made history | ||
}} | }} | ||
Revision as of 11:07, 10 June 2012
| Lardy HA, Wellman H (1952) Oxidative phosphorylations; rĂŽle of inorganic phosphate and acceptor systems in control of metabolic rates. J Biol Chem 195: 215-224. |
» PMID: 14938372; Open Access
Lardy HA, Wellman H (1952) J Biol Chem
Abstract: Rat liver mitochondria, prepared in 0.25 M sucrose and fortified with ATP, magnesium, and phosphate buffer, oxidize proline, glutamate, citrate, pyruvate, α-ketoglutarate, succinate, malate, and ÎČ-hydroxybutyrate at extremely slow rates. The rates of oxidation apparently are limited by the rate of transfer or hydrolysis of high energy phosphate compounds whose synthesis is coupled with the oxidative electron transport.
The rates of oxidation of all these substrates are greatly enhanced by phosphate acceptor systems such as adenylic acid, ADP, creatine plus its phosphorylating enzyme, or glucose plus hexokinase. With glucose and hexokinase as the acceptor system, P:O ratios of about 3 were obtained with glutamate, citrate, α-ketoglutarate, pyruvate, and ÎČ-hy- droxybutyrate as the substrate in systems in which the Krebs cycle of oxidations is proceeding.
2,4-Dinitrophenol, an agent which accelerates the breakdown of NP compounds, also accelerates the rate of oxygen consumption with each of the above substrates.
The initial rate of oxidation of caprylate to acetoacetate is enhanced by ~P acceptors. This oxidation resulted in P: 0 ratios of 1.1 to 1.4 with creatine as the ~P acceptor and of 1.7 to 2 with glucose as the ~P acceptor. âą Keywords: oxidative phosphorylation, phosphate acceptor, 2, 4-Dinitrophenol
Labels:
Organism: Rat
Tissue;cell: Hepatocyte; Liver"Hepatocyte; Liver" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.
Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
Made history
