Difference between revisions of "Lardy 1952 J Biol Chem"

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Lardy HA, Wellman H (1952) Oxidative phosphorylations; rôle of inorganic phosphate and acceptor systems in control of metabolic rates. J Biol Chem 195: 215-224.

» PMID: 14938372; Open Access

Lardy HA, Wellman H (1952) J Biol Chem

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 {{Publication
 |title=Lardy HA, Wellman H (1952) Oxidative phosphorylations; rôle of inorganic phosphate and acceptor systems in control of metabolic rates. J Biol Chem 195: 215-224.
 |info=[http://www.ncbi.nlm.nih.gov/pubmed/14938372 PMID: 14938372]; [http://www.jbc.org/content/195/1/215.full.pdf+html Open Access]
 |authors=Lardy HA, Wellman H
 |year=1952
 |journal=J Biol Chem
-|abstract=Rat  liver  mitochondria,  prepared  in  0.25  M  sucrose  and  fortified  with
-ATP,  magnesium,  and  phosphate  buffer,  oxidize  proline,  glutamate, citrate, pyruvate,  α-ketoglutarate,  succinate,  malate,  and  β-hydroxybutyrate  at extremely  slow  rates. The  rates  of  oxidation apparently  are  limited  by the  rate  of  transfer  or hydrolysis  of  high  energy  phosphate  compounds whose  synthesis  is coupled  with  the  oxidative  electron  transport.
-The  rates  of  oxidation  of  all  these  substrates  are  greatly  enhanced  by phosphate  acceptor  systems  such  as  adenylic  acid,  ADP,  creatine  plus
-its  phosphorylating  enzyme,  or  glucose  plus  hexokinase.  With  glucose
-and  hexokinase  as  the  acceptor  system,  P:O  ratios  of  about  3  were
-obtained  with  glutamate,  citrate,  α-ketoglutarate,  pyruvate,  and  β-hy-
-droxybutyrate  as  the  substrate  in  systems  in  which  the  Krebs  cycle  of
-oxidations  is  proceeding.
-,4-Dinitrophenol,  an  agent  which  accelerates  the  breakdown  of  NP compounds,  also  accelerates  the  rate  of  oxygen  consumption  with  each
-of  the  above  substrates.
-The  initial  rate  of  oxidation  of  caprylate  to  acetoacetate  is  enhanced by ~P  acceptors.  This  oxidation  resulted  in  P:  0  ratios  of  1.1  to  1.4 with  creatine  as  the  ~P  acceptor  and  of  1.7  to  2  with  glucose  as  the  ~P acceptor.
-|keywords=oxidative phosphorylation, phosphate acceptor, 2,4-Dinitrophenol
 }}
 {{Labeling
-|organism=Rat
-|tissues=Hepatocyte; Liver
-|preparations=Isolated Mitochondria
-|topics=Respiration; OXPHOS; ETS Capacity
 |additional=Made history
 }}