Difference between revisions of "Lisak 2015 Cell Death Differ"
(3 intermediate revisions by 2 users not shown) | |||
Line 1: | Line 1: | ||
{{Publication | {{Publication | ||
|title=Lisak D, Schacht T, Gawlitza A, Albrecht P, Aktas O, Koop B, Gliem M, Hofstetter HH, Zanger K, Bultynck G, Parys JB, De Smedt H, Kindler T, Adams-Quack P, Hahn M, Waisman A, Reed JC, Hövelmeyer N, Methner A (2015) BAX inhibitor-1 is a Ca<sup>2+</sup> channel critically important for immune cell function and survival. Cell Death Differ | |title=Lisak D, Schacht T, Gawlitza A, Albrecht P, Aktas O, Koop B, Gliem M, Hofstetter HH, Zanger K, Bultynck G, Parys JB, De Smedt H, Kindler T, Adams-Quack P, Hahn M, Waisman A, Reed JC, Hövelmeyer N, Methner A (2015) BAX inhibitor-1 is a Ca<sup>2+</sup> channel critically important for immune cell function and survival. Cell Death Differ 23:358-68. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/26470731 PMID: 26470731] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/26470731 PMID: 26470731] | ||
|authors=Lisak D, Schacht T, Gawlitza A, Albrecht P, Aktas O, Koop B, Gliem M, Hofstetter HH, Zanger K, Bultynck G, Parys JB, De Smedt H, Kindler T, Adams-Quack P, Hahn M, Waisman A, Reed JC, Hoevelmeyer N, Methner A | |authors=Lisak D, Schacht T, Gawlitza A, Albrecht P, Aktas O, Koop B, Gliem M, Hofstetter HH, Zanger K, Bultynck G, Parys JB, De Smedt H, Kindler T, Adams-Quack P, Hahn M, Waisman A, Reed JC, Hoevelmeyer N, Methner A | ||
Line 10: | Line 10: | ||
{{Labeling | {{Labeling | ||
|area=Respiration, Genetic knockout;overexpression | |area=Respiration, Genetic knockout;overexpression | ||
|diseases=Obesity | |||
|organism=Mouse | |organism=Mouse | ||
|tissues=Blood cells | |tissues=Blood cells, Lymphocyte | ||
|preparations=Intact cells | |preparations=Intact cells | ||
|topics=Calcium | |topics=Calcium | ||
|couplingstates=LEAK, ROUTINE, | |couplingstates=LEAK, ROUTINE, ET | ||
| | |pathways=ROX | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
}} | }} |
Latest revision as of 15:11, 13 November 2017
Lisak D, Schacht T, Gawlitza A, Albrecht P, Aktas O, Koop B, Gliem M, Hofstetter HH, Zanger K, Bultynck G, Parys JB, De Smedt H, Kindler T, Adams-Quack P, Hahn M, Waisman A, Reed JC, Hövelmeyer N, Methner A (2015) BAX inhibitor-1 is a Ca2+ channel critically important for immune cell function and survival. Cell Death Differ 23:358-68. |
Lisak D, Schacht T, Gawlitza A, Albrecht P, Aktas O, Koop B, Gliem M, Hofstetter HH, Zanger K, Bultynck G, Parys JB, De Smedt H, Kindler T, Adams-Quack P, Hahn M, Waisman A, Reed JC, Hoevelmeyer N, Methner A (2015) Cell Death Differ
Abstract: The endoplasmic reticulum (ER) serves as the major intracellular Ca2+ store and has a role in the synthesis and folding of proteins. BAX (BCL2-associated X protein) inhibitor-1 (BI-1) is a Ca2+ leak channel also implicated in the response against protein misfolding, thereby connecting the Ca2+ store and protein-folding functions of the ER. We found that BI-1-deficient mice suffer from leukopenia and erythrocytosis, have an increased number of splenic marginal zone B cells and higher abundance and nuclear translocation of NF-κB (nuclear factor-κ light-chain enhancer of activated B cells) proteins, correlating with increased cytosolic and ER Ca2+ levels. When put into culture, purified knockout T cells and even more so B cells die spontaneously. This is preceded by increased activity of the mitochondrial initiator caspase-9 and correlated with a significant surge in mitochondrial Ca2+ levels, suggesting an exhausted mitochondrial Ca2+ buffer capacity as the underlying cause for cell death in vitro. In vivo, T-cell-dependent experimental autoimmune encephalomyelitis and B-cell-dependent antibody production are attenuated, corroborating the ex vivo results. These results suggest that BI-1 has a major role in the functioning of the adaptive immune system by regulating intracellular Ca2+ homeostasis in lymphocytes.
• O2k-Network Lab: DE Mainz Methner A
Labels: MiParea: Respiration, Genetic knockout;overexpression
Pathology: Obesity
Organism: Mouse Tissue;cell: Blood cells, Lymphocyte Preparation: Intact cells
Regulation: Calcium Coupling state: LEAK, ROUTINE, ET Pathway: ROX HRR: Oxygraph-2k