Difference between revisions of "Lyon 2006 Anal Chem"
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|title=Lyon J L, Stevenson K J (2006) Picomolar peroxide detection using a chemically activated redox mediator and square wave voltammetry. Anal Chem 78: 8518- | |title=Lyon J L, Stevenson K J (2006) Picomolar peroxide detection using a chemically activated redox mediator and square wave voltammetry. Anal Chem 78:8518-25. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/17165848 PMID: 17165848] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/17165848 PMID: 17165848] | ||
|authors=Lyon J L, Stevenson K J | |authors=Lyon J L, Stevenson K J | ||
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{{Labeling | {{Labeling | ||
|area=Instruments;methods | |area=Instruments;methods | ||
|injuries=RONS | |injuries=Oxidative stress;RONS | ||
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Latest revision as of 16:38, 23 February 2015
Lyon J L, Stevenson K J (2006) Picomolar peroxide detection using a chemically activated redox mediator and square wave voltammetry. Anal Chem 78:8518-25. |
Lyon J L, Stevenson K J (2006) Anal Chem
Abstract: A method for low-level, low-potential electrochemical detection of hydrogen peroxide using a chemically activated redox mediator is presented. This method is unique in that it utilizes a mediator, Amplex Red, which is only redox-active when chemically oxidized by H2O2 in the presence of the enzyme horseradish peroxidase (HRP). When employed in concert with microelectrode square wave voltammetry to optimize sensing at ultralow concentrations (<1 microM), this method exhibits marked improvements in analytical sensitivity and detection limits (limit of detection as low as 8 pM) over existing protocols. Sensing schemes incorporating both freely diffusing and immobilized HRP are evaluated, and the resulting analytical sensitivities are 1.22 +/- 0.04 and (2.1 +/- 0.6) x 10(-1) microA/(microM mm2), respectively, for peroxide concentrations in the high picomolar to low micromolar range. A second linear region exists for lower peroxide concentrations. Furthermore, quantitative enzyme kinetics analysis using Michaelis-Menten parameters is possible through interpretation of data collected in this scheme. Km values for soluble and immobilized HRP were 84 +/- 13 and 504 +/- 19 microM, respectively. This method is amenable to any biological detection scheme that generates hydrogen peroxide as a reactive product. β’ Keywords: Amplex Red
Labels: MiParea: Instruments;methods
Stress:Oxidative stress;RONS