Difference between revisions of "Malonate"
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|type=Inhibitor ETS | |type=Inhibitor ETS | ||
}} | }} | ||
== Application in [[HRR]] == | == Application in [[HRR]] == | ||
'''Mna: Malonate''' (Malonic acid, CH<sub>2</sub>(COOH)<sub>2</sub>); Sigma M 1296, 5 g, store at -20 °C: FW = 800) | '''Mna: Malonate''' (Malonic acid, CH<sub>2</sub>(COOH)<sub>2</sub>); Sigma M 1296, 5 g, store at -20 °C: FW = 800) | ||
<span style="color:#8B008B"> '''Caution:''' | <span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be adjusted to room temperature before opening.</span> | ||
'''Preparation of | '''Preparation of 1 mM stock solution''' (dissolved in Ethanol): | ||
::1) weigh | ::1) weigh 1.27 mg of FCCP directly into a 5 ml glass vial | ||
::2) add | ::2) add 5 ml EtOH | ||
::3) divide into 0.2 ml portions | ::3) divide into 0.2 ml portions OR store in 5 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution | ||
::4) store | ::4) store in glass vials (recommendation) at -20 °C | ||
'''Oxygraph-2k Manual Titrations:''' [[MiPNet09.12]] | '''Oxygraph-2k Manual Titrations:''' [[MiPNet09.12]] |
Revision as of 14:25, 22 November 2010
Description
Malonate (malonic acid) is a competitive inhibitor of Succinate dehydrogenase (Complex II).
Abbreviation: Mna
Reference: MiPNet09.12
Labels:
Enzyme: Complex II; Succinate Dehydrogenase"Complex II; Succinate Dehydrogenase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property.
HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
Application in HRR
Mna: Malonate (Malonic acid, CH2(COOH)2); Sigma M 1296, 5 g, store at -20 °C: FW = 800)
Caution: Chemicals stored in the fridge or freezer should be adjusted to room temperature before opening.
Preparation of 1 mM stock solution (dissolved in Ethanol):
- 1) weigh 1.27 mg of FCCP directly into a 5 ml glass vial
- 2) add 5 ml EtOH
- 3) divide into 0.2 ml portions OR store in 5 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution
- 4) store in glass vials (recommendation) at -20 °C
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 1 µl using a 10 µl syringe
- Final conc. in 2 ml O2k-chamber: 2 µg/ml