Difference between revisions of "MiPNet08.18 LactateDehydrogenase"
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{{Publication | {{Publication | ||
|title=Kuznetsov AV, Gnaiger E. Laboratory protocol: Lactate dehydrogenase. Cytosolic marker enzyme. Mitochondr. Physiol. Network 08.18. | |title=Kuznetsov AV, Gnaiger E. Laboratory protocol: Lactate dehydrogenase. Cytosolic marker enzyme. Mitochondr. Physiol. Network 08.18. | ||
|info=[http://www.oroboros.at/index.php?id=protocosl_ldh MiPNet08.18] | |||
|authors=Kuznetsov AV, Gnaiger E | |authors=Kuznetsov AV, Gnaiger E | ||
|year=* | |year=* | ||
|journal=Mitochondr. Physiol. Network | |journal=Mitochondr. Physiol. Network | ||
|abstract=Lactate dehydrogenase (EC 1.1.1.27) is an enzyme, which catalyzes the last step in glycolysis. LDH is a soluble enzyme and localized in the cytosol (cytoplasm). LDH, therefore, is used as a quantitative marker enzyme for the intact cell, its activity providing information on cellular glycolytic capacity (Renner et al., 2003). Measurement of LDH release (leakage) is an important and frequently applied test for cellular membrane permeabilization (rupture) and severe irreversible cell damage. LDH leakage normally correlates well with CK release and the trypan blue viability test. | |||
|keywords=Enzymes | |||
|mipnetlab=AT_Innsbruck_OROBOROS | |mipnetlab=AT_Innsbruck_OROBOROS | ||
| | |articletype=Protocol; Manual, MiPNet-online Publication | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|instruments=Oxygraph-2k, Protocol, MiPNet-Publication | |||
|preparations=Enzyme | |preparations=Enzyme | ||
|articletype=Protocol; Manual, MiPNet-online Publication | |articletype=Protocol; Manual, MiPNet-online Publication | ||
}} | }} | ||
Revision as of 17:12, 13 May 2011
| Kuznetsov AV, Gnaiger E. Laboratory protocol: Lactate dehydrogenase. Cytosolic marker enzyme. Mitochondr. Physiol. Network 08.18. |
) Mitochondr. Physiol. Network
Abstract: Lactate dehydrogenase (EC 1.1.1.27) is an enzyme, which catalyzes the last step in glycolysis. LDH is a soluble enzyme and localized in the cytosol (cytoplasm). LDH, therefore, is used as a quantitative marker enzyme for the intact cell, its activity providing information on cellular glycolytic capacity (Renner et al., 2003). Measurement of LDH release (leakage) is an important and frequently applied test for cellular membrane permeabilization (rupture) and severe irreversible cell damage. LDH leakage normally correlates well with CK release and the trypan blue viability test. • Keywords: Enzymes
• O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels:
Preparation: Enzyme
HRR: Oxygraph-2k, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., MiPNet-Publication"MiPNet-Publication" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
