MiPNet12.23 FiberRespiration: Difference between revisions
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{{Publication | {{Publication | ||
|title=Lemieux H, Votion DM, Gnaiger E (2007) Mitochondrial respiration in permeabilized fibres: Needle biopsies from horse skeletal muscle. Mitochondr Physiol Network 12.23. | |title=Lemieux H, Votion DM, Gnaiger E (2007) Mitochondrial respiration in permeabilized fibres: Needle biopsies from horse skeletal muscle. Mitochondr Physiol Network 12.23. | ||
|info='''Open Access - [ | |info=[[Media:MiPNet12.23 FibreRespiration.pdf|'''Open Access-MiPNet12.23.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet12.23_FibreRespiration.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]] | ||
|authors=OROBOROS | |authors=OROBOROS | ||
|year=2007 | |year=2007 | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|abstract=Methodological and conceptual features of highresolution respirometry are illustrated in an experiment with permeabilized fibres in the OROBOROS Oxygraph- | |abstract='''O2k-Protocol for Mitochondrial Respiration in Permeabilized Fibres: Needle Biopsies from Horse Skeletal Muscle''' | ||
Methodological and conceptual features of highresolution respirometry are illustrated in an experiment with permeabilized fibres in the OROBOROS Oxygraph- | |||
2k (O2k). The experiment demonstrates manual titrations applied to study respiratory capacity in permeabilized fibres. Application of the DatLab 4 | 2k (O2k). The experiment demonstrates manual titrations applied to study respiratory capacity in permeabilized fibres. Application of the DatLab 4 | ||
software is shown for on-line data analysis [[MiPNet12.09]] The following guideline describes the experimental protocol and includes a short discussion of | software is shown for on-line data analysis [[MiPNet12.09]] The following guideline describes the experimental protocol and includes a short discussion of | ||
results. The experiments was carried out by participants of an O2k-Course on HRR in December 2007 (IOC44; Schroecken, Austria). | results. The experiments was carried out by participants of an O2k-Course on HRR in December 2007 (IOC44; Schroecken, Austria). | ||
:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents] | |||
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]] | |||
|mipnetlab=AT_Innsbruck_OROBOROS, BE_Liege_Votion DM | |mipnetlab=AT_Innsbruck_OROBOROS, BE_Liege_Votion DM | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration, Instruments;methods | |area=Respiration, Instruments;methods | ||
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|instruments=Oxygraph-2k, Protocol | |instruments=Oxygraph-2k, Protocol | ||
|additional=O2k-Demo | |additional=O2k-Demo | ||
}} | }} | ||
== | |||
MiPNet04.07 is integrated in MiPNet12.23. | == Further information == | ||
:>> [http://www.oroboros.at/?protocols_muscle_horse MiPNet12.23] | |||
:>> MiPNet04.07 is integrated in MiPNet12.23. | |||
* Version 1: 2007 | * Version 1: 2007 | ||
* Version 6: 2011-12-11 | * Version 6: 2011-12-11 | ||
Revision as of 08:34, 18 October 2013
| Lemieux H, Votion DM, Gnaiger E (2007) Mitochondrial respiration in permeabilized fibres: Needle biopsies from horse skeletal muscle. Mitochondr Physiol Network 12.23. |
» Open Access-MiPNet12.23.pdf; Versions
OROBOROS (2007) Mitochondr Physiol Network
Abstract: O2k-Protocol for Mitochondrial Respiration in Permeabilized Fibres: Needle Biopsies from Horse Skeletal Muscle
Methodological and conceptual features of highresolution respirometry are illustrated in an experiment with permeabilized fibres in the OROBOROS Oxygraph- 2k (O2k). The experiment demonstrates manual titrations applied to study respiratory capacity in permeabilized fibres. Application of the DatLab 4 software is shown for on-line data analysis MiPNet12.09 The following guideline describes the experimental protocol and includes a short discussion of results. The experiments was carried out by participants of an O2k-Course on HRR in December 2007 (IOC44; Schroecken, Austria).
- >> O2k-Protocols:Overall contents
- >> Product: OROBOROS Oxygraph-2k, O2k-Catalogue
• O2k-Network Lab: AT_Innsbruck_OROBOROS, BE_Liege_Votion DM
Labels: MiParea: Respiration, Instruments;methods
Organism: Horse
Tissue;cell: Skeletal muscle
Preparation: Permeabilized tissue
Regulation: Coupling efficiency;uncoupling, Cyt c Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-Demo
Further information
- >> MiPNet12.23
- >> MiPNet04.07 is integrated in MiPNet12.23.
- Version 1: 2007
- Version 6: 2011-12-11
