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| {{Mainpage OROBOROS}}
| | #REDIRECT [[MiPNet19.18D O2k-Series G and DatLab 6: Calibration]] |
| {{Publication
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| |title=[[Image:O2k-Manual.jpg|right|70px|link=http://wiki.oroboros.at/index.php/O2k-Manual|O2k-Manual]] Oxygen calibration by DatLab.
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| |info=[[Media:MiPNet19.01D O2k-Calibration.pdf|'''Open Access-MiPNet19.01D.pdf''']], [http://www.bioblast.at/index.php/File:MiPNet19.01D_O2k-Calibration.pdf Versions]
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| |authors=OROBOROS
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| |year=2014-03
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| |journal=Mitochondr Physiol Network
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| |abstract='''Gnaiger E (2014) Oxygen calibration by DatLab. Mitochondr Physiol Network 19.01(D01): 41-48.'''
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| [[Accuracy|Accurate]] calibration of the oxygen sensor depends on (1) calibration solutions prepared at known oxygen partial pressures, as achieved in the [[Oxygraph-2k]] (O2k) at defined temperature, continuously recorded total gas pressure (barometric pressure), and thermodynamic equilibrium between the gas and aqueous phase; (2) high stability of the signal of the [[polarographic oxygen sensor]] (POS), tested for sufficiently long periods of time; (3) linearity of signal output with oxygen pressure, achieved with the POS in the range between oxygen saturation and zero oxygen pressure; and (4) application of accurate [[oxygen solubility]] for aqueous solutions for the conversion of partial [[oxygen pressure]] into [[oxygen concentration]] ([[MiPNet06.03]]). The standard oxygen calibration procedure is described for O2k high-resolution respirometry with the automatic calibration routine of DatLab.
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| :>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]
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| |keywords=HRR, DatLab
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| |mipnetlab=AT_Innsbruck_OROBOROS
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| }}
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| {{Labeling
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| |area=Instruments;methods
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| |instruments=Oxygraph-2k, O2k-Manual, Protocol
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| |additional=O2k-Core, DatLab
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| }}
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| == Related O2k-SOP ==
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| :: >> [[MiPNet06.03 POS-Calibration-SOP]] | |
| :: >> [[MiPNet14.06 InstrumentalBackground]]
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| [[Category:OroboPedia]]
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