Difference between revisions of "MiPNet19.18D O2k-Series G and DatLab 6: Calibration"
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MiPNet08.07, MiPNet09.08 and MiPNet02.01 (DatLab 2) is integrated into MiPNet12.08. | MiPNet08.07, MiPNet09.08 and MiPNet02.01 (DatLab 2) is integrated into MiPNet12.08. | ||
Revision as of 17:37, 13 May 2011
| Gnaiger E. Oxygen and pX Calibration DatLab4.3. Mitochondr. Physiol. Network 12.08. |
» MiPNet12.08
) Mitochondr. Physiol. Network
Abstract: Accurate calibration of the oxygen sensor depends on (1) calibration solutions prepared at known oxygen partial pressures, as achieved in the Oxygraph-2k (O2k) at defined temperature, continuously recorded total gas pressure (barometric pressure), and thermodynamic equilibrium between the gas and aqueous phase; (2) high stability of the signal of the polarographic oxygen sensor (POS), tested for sufficiently long periods of time; (3) linearity of signal output with oxygen pressure, achieved with the POS in the range between oxygen saturation and zero oxygen pressure; and (4) application of accurate oxygen solubilities for aqueous solutions for the conversion of partial oxygen pressure into oxygen concentration MiPNet06.03. The standard oxygen calibration procedure is described for O2k high-resolution respirometry with the automatic calibration routine of DatLab 4. • Keywords: HRR-DatLab
• O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels:
HRR: Oxygraph-2k, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., MiPNet-Publication"MiPNet-Publication" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
Info
MiPNet08.07, MiPNet09.08 and MiPNet02.01 (DatLab 2) is integrated into MiPNet12.08.
