Difference between revisions of "MiPNet19.18D O2k-Series G and DatLab 6: Calibration"
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{{Publication | {{Publication | ||
|title=Gnaiger E. Oxygen and pX Calibration | |title=Gnaiger E. Oxygen and pX Calibration DatLab 4.3. Mitochondr Physiol Network 12.08. | ||
|info=[http://www.oroboros.at/index.php? | |info=[http://www.oroboros.at/index.php?o2k-o2calibration MiPNet12.08] | ||
|authors=Gnaiger E | |authors=Gnaiger E | ||
|year=* | |year=* | ||
|journal=Mitochondr | |journal=Mitochondr Physiol Network | ||
|abstract=Accurate calibration of the oxygen sensor depends on (1) | |abstract=[[Accuracy|Accurate]] calibration of the oxygen sensor depends on (1) | ||
calibration solutions prepared at known oxygen partial pressures, as | calibration solutions prepared at known oxygen partial pressures, as | ||
achieved in the Oxygraph-2k (O2k) at defined temperature, continuously | achieved in the [[Oxygraph-2k]] (O2k) at defined temperature, continuously | ||
recorded total gas pressure (barometric pressure), and thermodynamic | recorded total gas pressure (barometric pressure), and thermodynamic | ||
equilibrium between the gas and aqueous phase; (2) high stability of the | equilibrium between the gas and aqueous phase; (2) high stability of the | ||
signal of the polarographic oxygen sensor (POS), tested for sufficiently | signal of the [[polarographic oxygen sensor]] (POS), tested for sufficiently | ||
long periods of time; (3) linearity of signal output with oxygen pressure, | long periods of time; (3) linearity of signal output with oxygen pressure, | ||
achieved with the POS in the range between oxygen saturation and zero | achieved with the POS in the range between oxygen saturation and zero | ||
oxygen pressure; and (4) application of accurate oxygen | oxygen pressure; and (4) application of accurate [[oxygen solubility]] for | ||
aqueous solutions for the conversion of partial oxygen pressure into | aqueous solutions for the conversion of partial [[oxygen pressure]] into | ||
oxygen concentration [[MiPNet06.03]]. The standard oxygen calibration | [[oxygen concentration]] ([[MiPNet06.03]]). The standard oxygen calibration | ||
procedure is described for O2k high-resolution respirometry with the | procedure is described for O2k high-resolution respirometry with the | ||
automatic calibration routine of DatLab 4. | automatic calibration routine of DatLab 4. | ||
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==Info== | ==Info== | ||
MiPNet08.07, MiPNet09.08 and MiPNet02.01 (DatLab 2) | MiPNet08.07, MiPNet09.08 and MiPNet02.01 (DatLab 2) are integrated into MiPNet12.08. | ||
Revision as of 02:21, 4 December 2011
| Gnaiger E. Oxygen and pX Calibration DatLab 4.3. Mitochondr Physiol Network 12.08. |
Abstract: Accurate calibration of the oxygen sensor depends on (1) calibration solutions prepared at known oxygen partial pressures, as achieved in the Oxygraph-2k (O2k) at defined temperature, continuously recorded total gas pressure (barometric pressure), and thermodynamic equilibrium between the gas and aqueous phase; (2) high stability of the signal of the polarographic oxygen sensor (POS), tested for sufficiently long periods of time; (3) linearity of signal output with oxygen pressure, achieved with the POS in the range between oxygen saturation and zero oxygen pressure; and (4) application of accurate oxygen solubility for aqueous solutions for the conversion of partial oxygen pressure into oxygen concentration (MiPNet06.03). The standard oxygen calibration procedure is described for O2k high-resolution respirometry with the automatic calibration routine of DatLab 4. • Keywords: HRR-DatLab
• O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels:
HRR: Oxygraph-2k, O2k-Manual, MiPNet-Publication"MiPNet-Publication" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
Info
MiPNet08.07, MiPNet09.08 and MiPNet02.01 (DatLab 2) are integrated into MiPNet12.08.
