MiPNet19.18D O2k-Series G and DatLab 6: Calibration

Gnaiger E (2013) Oxygen and pX (pH) Calibration by DatLab. Mitochondr Physiol Network 12.08.

» Bioblast File:MiPNet19.01D O2k-Calibration.pdf, MiPNet12.08 Open Access

OROBOROS (2013) Mitochondr Physiol Network

Abstract: O2k-Manual Chapter D - O2k-Manual contents

Accurate calibration of the oxygen sensor depends on (1) calibration solutions prepared at known oxygen partial pressures, as achieved in the Oxygraph-2k (O2k) at defined temperature, continuously recorded total gas pressure (barometric pressure), and thermodynamic equilibrium between the gas and aqueous phase; (2) high stability of the signal of the polarographic oxygen sensor (POS), tested for sufficiently long periods of time; (3) linearity of signal output with oxygen pressure, achieved with the POS in the range between oxygen saturation and zero oxygen pressure; and (4) application of accurate oxygen solubility for aqueous solutions for the conversion of partial oxygen pressure into oxygen concentration (MiPNet06.03). The standard oxygen calibration procedure is described for O2k high-resolution respirometry with the automatic calibration routine of DatLab. • Keywords: HRR, DatLab

• O2k-Network Lab: AT_Innsbruck_OROBOROS

Labels: MiParea: Instruments;methods 

HRR: Oxygraph-2k, O2k-Manual, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property. 

O2k-SOP, DatLab 

Info

• Version 6: 2012-10-24
• Version 1: 2007
• MiPNet08.07, MiPNet09.08 and MiPNet02.01 (DatLab 2) are integrated into MiPNet12.08.