Difference between revisions of "MiPNet19.18D O2k-Series G and DatLab 6: Calibration"
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oxygen pressure; and (4) application of accurate [[oxygen solubility]] for | oxygen pressure; and (4) application of accurate [[oxygen solubility]] for | ||
aqueous solutions for the conversion of partial [[oxygen pressure]] into | aqueous solutions for the conversion of partial [[oxygen pressure]] into | ||
oxygen concentration ([[MiPNet06.03]]). The standard oxygen calibration | [[oxygen concentration]] ([[MiPNet06.03]]). The standard oxygen calibration | ||
procedure is described for O2k high-resolution respirometry with the | procedure is described for O2k high-resolution respirometry with the | ||
automatic calibration routine of DatLab. | automatic calibration routine of DatLab. | ||
| Line 30: | Line 30: | ||
|instruments=Oxygraph-2k, O2k-Manual, Protocol | |instruments=Oxygraph-2k, O2k-Manual, Protocol | ||
|additional=O2k-SOP, DatLab | |additional=O2k-SOP, DatLab | ||
}} | }} | ||
== Further information == | == Further information == | ||
:>> [http://www.oroboros.at/index.php?O2k-o2calibration MiPNet12.08] | :>> [http://www.oroboros.at/index.php?O2k-o2calibration MiPNet12.08] | ||
Revision as of 10:01, 17 October 2013
| Gnaiger E (2013) Oxygen and pX (pH) Calibration by DatLab. Mitochondr Physiol Network 12.08. |
» Open Access-MiPNet12.08.pdf, Versions
OROBOROS (2013) Mitochondr Physiol Network
Abstract: O2k-Manual Chapter D
Accurate calibration of the oxygen sensor depends on (1) calibration solutions prepared at known oxygen partial pressures, as achieved in the Oxygraph-2k (O2k) at defined temperature, continuously recorded total gas pressure (barometric pressure), and thermodynamic equilibrium between the gas and aqueous phase; (2) high stability of the signal of the polarographic oxygen sensor (POS), tested for sufficiently long periods of time; (3) linearity of signal output with oxygen pressure, achieved with the POS in the range between oxygen saturation and zero oxygen pressure; and (4) application of accurate oxygen solubility for aqueous solutions for the conversion of partial oxygen pressure into oxygen concentration (MiPNet06.03). The standard oxygen calibration procedure is described for O2k high-resolution respirometry with the automatic calibration routine of DatLab.
• Keywords: HRR, DatLab
• O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels: MiParea: Instruments;methods
HRR: Oxygraph-2k, O2k-Manual, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-SOP, DatLab
Further information
- >> MiPNet12.08
- >> MiPNet08.07, MiPNet09.08 and MiPNet02.01 (DatLab 2) are integrated into MiPNet12.08.
- Version 6: 2012-10-24
- Version 1: 2007
