MiPNet20.13 Safranin mt-membranepotential

Safranin data acquisition and analysis (2014) O2k-Fluorometry: HRR and simultaneous determination of mt-membrane potential with safranin.

» Open Access-MiPNet19.19.pdf; Versions

OROBOROS (2014-10) Mitochondr Physiol Network

Abstract: Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) O2k-Fluorometry: HRR and simultaneous determination of mt-membrane potential with safranin. Mitochondr Physiol Network 19.19(01): 1-5.

• O2k-Network Lab: AT_Innsbruck_OROBOROS

Labels: MiParea: Respiration, Instruments;methods 

Organism: Mouse  Tissue;cell: Nervous system  Preparation: Homogenate 

Coupling state: LEAK, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property. 

HRR: Oxygraph-2k, O2k-Fluorometer, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property. 

Reference

• Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542: 163-81. » PMID: 24862266

• Safranin used in the experiments for this publication was Safranin O from Sigma (# S2255, 25 g).