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Difference between revisions of "MiPNet20.13 Safranin mt-membranepotential"

From Bioblast
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{{Publication
{{Publication
|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] Safranin data acquisition and analysis (2014) O2k-Fluorometry: HRR and simultaneous determination of mt-membrane potential with safranin.
|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] Safranin data acquisition and analysis (2014) O2k-Fluorometry: HRR and simultaneous determination of mt-membrane potential with safranin. [[Media: MiPNet19.19_Safranin_DataAcquisitionAnalysis.pdf |»Bioblast pdf«]]
|info=[[Media: MiPNet19.19_Safranin_DataAcquisitionAnalysis.pdf |'''Open Access-MiPNet19.19.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet19.19_Safranin_DataAcquisitionAnalysis.pdf Versions]
|info=[http://www.bioblast.at/index.php/File:MiPNet19.19_Safranin_DataAcquisitionAnalysis.pdf Versions]
|authors=OROBOROS
|authors=OROBOROS
|year=2015-02-05
|year=2015-02-05
|journal=Mitochondr Physiol Network
|journal=Mitochondr Physiol Network
|abstract='''Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) O2k-Fluorometry: HRR and simultaneous determination of mt-membrane potential with safranin. Mitochondr Physiol Network 19.19(01): 1-5.'''
|abstract=Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) O2k-Fluorometry: HRR and simultaneous determination of mt-membrane potential with safranin. Mitochondr Physiol Network 19.19(01):1-5.
|mipnetlab=AT_Innsbruck_OROBOROS
|mipnetlab=AT_Innsbruck_OROBOROS
}}
}}

Revision as of 13:52, 20 May 2015

Publications in the MiPMap
O2k-Protocols
Safranin data acquisition and analysis (2014) O2k-Fluorometry: HRR and simultaneous determination of mt-membrane potential with safranin. »Bioblast pdf«

» Versions

OROBOROS (2015-02-05) Mitochondr Physiol Network

Abstract: Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) O2k-Fluorometry: HRR and simultaneous determination of mt-membrane potential with safranin. Mitochondr Physiol Network 19.19(01):1-5.


O2k-Network Lab: AT_Innsbruck_OROBOROS


Labels: MiParea: Respiration, Instruments;methods 


Organism: Mouse  Tissue;cell: Nervous system  Preparation: Homogenate 


Coupling state: LEAK, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property. 

HRR: Oxygraph-2k, O2k-Fluorometer, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property. 

O2k-Demo, O2k-MultiSensor 

Reference

  • Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542: 163-81. » PMID: 24862266
  • Safranin used in the experiments for this publication was Safranin O from Sigma (# S2255, 25 g).