Difference between revisions of "MiPNet21.07 MitoFit DatLab PT"
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===Abstract:=== | |||
The MitoFit DatLab analysis test can be considered as a first-level 'dry' MitoFit Proficiency Test for evaluation of inter-individual and inter-laboratory reproducibility and accuracy of DatLab analysis of the same data files. Proficiency will be tested for (1) setting Marks for analysis of flux; (2) O2 calibration at air saturation and zero O2 concentration; (3) analysis of instrumental O2 background and copying of parameters for flux analysis. | |||
===Aims=== | |||
# Obtain a measure of noise due to variability in setting marks on 'flux' when evaluating flux in different respiratory states. | |||
# Guide the user to the DatLab-Excel template for analysis. | |||
# Proficiency test: Two-point O<sub>2</sub> calibration, with O<sub>2</sub> solubility factor. | |||
# Obtain a measure of noise of instrumental O<sub>2</sub> background parameters due to variability in setting marks on 'flux'. | |||
# Proficiency test: Application of instrumental O<sub>2</sub> background to analysis of flux. | |||
===Files to download:=== | |||
# Instructions: [[File:DatLabanalysisTest_instructions.pdf]] | |||
# Experimental data file: [[File:file_exp1_xx.DLD]] | |||
# Template: [[File:Template1 xx.xls]] | |||
# Calibration and Background file: [[File:file_bgr1_xx.DLD]] | |||
Revision as of 11:34, 28 January 2016
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» Versions
OROBOROS (2016-01-21) Mitochondr Physiol Network
Abstract: Krumschnabel G, Plattner C, Gnaiger (2016) MitoFit DatLab proficiency test. Mitochondr Physiol Network 21.07(01):1-##.
- » Instrument: O2k, DatLab, O2k-Catalogue
- Page in prep.
• O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels: MiParea: Instruments;methods
Organism: Human
Preparation: Permeabilized cells
Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, O2k-Protocol
O2k-SOP, MitoFitPublication
Abstract:
The MitoFit DatLab analysis test can be considered as a first-level 'dry' MitoFit Proficiency Test for evaluation of inter-individual and inter-laboratory reproducibility and accuracy of DatLab analysis of the same data files. Proficiency will be tested for (1) setting Marks for analysis of flux; (2) O2 calibration at air saturation and zero O2 concentration; (3) analysis of instrumental O2 background and copying of parameters for flux analysis.
Aims
- Obtain a measure of noise due to variability in setting marks on 'flux' when evaluating flux in different respiratory states.
- Guide the user to the DatLab-Excel template for analysis.
- Proficiency test: Two-point O2 calibration, with O2 solubility factor.
- Obtain a measure of noise of instrumental O2 background parameters due to variability in setting marks on 'flux'.
- Proficiency test: Application of instrumental O2 background to analysis of flux.
Files to download:
- Instructions: File:DatLabanalysisTest instructions.pdf
- Experimental data file: File:File exp1 xx.DLD
- Template: File:Template1 xx.xls
- Calibration and Background file: File:File bgr1 xx.DLD
