Difference between revisions of "MiPNet21.07 MitoFit DatLab PT"
| Line 38: | Line 38: | ||
# DatLab proficiency test instructions: [[Media:DatLabanalysisTest_instructions.pdf|Instructions]] | # DatLab proficiency test instructions: [[Media:DatLabanalysisTest_instructions.pdf|Instructions]] | ||
# Experimental data file: [[Media:file_exp1_xx.DLD| | # Experimental data file: [[Media:file_exp1_xx.DLD|2015-09-24 P1-01.DLD]] | ||
# Template: [[Media:Template1 xx.xls| | # Template: [[Media:Template1 xx.xls|NS 1PM 2D 3G 4c 5S 6U 7Rot 8Ama.xls]] | ||
# Calibration and Background file: [[Media:file_bgr1_xx.DLD| | # Calibration and Background file: [[Media:file_bgr1_xx.DLD|2015-03-30 P1-01.DLD]] | ||
Revision as of 17:35, 16 February 2016
 |
» Versions
OROBOROS (2016-01-21) Mitochondr Physiol Network
Abstract: Krumschnabel G, Plattner C, Gnaiger (2016) MitoFit DatLab proficiency test. Mitochondr Physiol Network 21.07(01):1-##.
The MitoFit DatLab analysis test can be considered as a first-level 'dry' MitoFit Proficiency Test for evaluation of inter-individual and inter-laboratory reproducibility and accuracy of DatLab analysis of the same data files. Proficiency will be tested for (1) setting Marks for analysis of flux; (2) O2 calibration at air saturation and zero O2 concentration; (3) analysis of instrumental O2 background.
- » DatLab
• O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels: MiParea: Instruments;methods
Organism: Human
Preparation: Permeabilized cells
Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, O2k-Protocol
MitoFitPublication
Aims
- Obtain a measure of noise due to variability in setting marks on 'flux' when evaluating flux in different respiratory states.
- Guide the user to the DatLab-Excel template for analysis.
- Proficiency test: Two-point O2 calibration, with O2 solubility factor.
- Obtain a measure of noise of instrumental O2 background parameters due to variability in setting marks on 'flux'.
- Proficiency test: Application of instrumental O2 background to analysis of flux.
Files to download:
- DatLab proficiency test instructions: Instructions
- Experimental data file: 2015-09-24 P1-01.DLD
- Template: NS 1PM 2D 3G 4c 5S 6U 7Rot 8Ama.xls
- Calibration and Background file: 2015-03-30 P1-01.DLD
