Difference between revisions of "MiPNet21.07 MitoFit DatLab PT"
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{{Publication | {{Publication | ||
|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] MitoFit DatLab proficiency test. - | |title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] MitoFit DatLab proficiency test. - | ||
|info=[[File:PDF.jpg|100px|link= http://wiki.oroboros.at/index.php?title=Special:Upload&wpDestFile=MiPNet21.07_MitoFit_DatLab_proficiency_test.pdf|Bioblast pdf]] | |info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/index.php?title=Special:Upload&wpDestFile=MiPNet21.07_MitoFit_DatLab_proficiency_test.pdf|Bioblast pdf]] | ||
|authors=OROBOROS | |authors=OROBOROS | ||
|year=2016-01-21 | |year=2016-01-21 | ||
Revision as of 15:48, 9 March 2016
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OROBOROS (2016-01-21) Mitochondr Physiol Network
Abstract: Krumschnabel G, Plattner C, Gnaiger (2016) MitoFit DatLab proficiency test. Mitochondr Physiol Network 21.07(01):1-##. Versions
The MitoFit DatLab analysis test can be considered as a first-level 'dry' MitoFit Proficiency Test for evaluation of inter-individual and inter-laboratory reproducibility and accuracy of DatLab analysis of the same data files. Proficiency will be tested for (1) setting Marks for analysis of flux; (2) O2 calibration at air saturation and zero O2 concentration; (3) analysis of instrumental O2 background.
- » DatLab
• O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels: MiParea: Instruments;methods
Organism: Human
Preparation: Permeabilized cells
Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, O2k-Protocol
MitoFitPublication
Aims
- Obtain a measure of noise due to variability in setting marks on 'flux' when evaluating flux in different respiratory states.
- Guide the user to the DatLab-Excel template for analysis.
- Proficiency test: Two-point O2 calibration, with O2 solubility factor.
- Obtain a measure of noise of instrumental O2 background parameters due to variability in setting marks on 'flux'.
Files to download:
- DatLab proficiency test instructions: Instructions
- Experimental data file: 2015-09-24 P1-01.DLD
- Template: NS 1PM 2D 3G 4c 5S 6U 7Rot 8Ama.xls
- Calibration and Background file: 2015-03-30 P1-01.DLD
