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Difference between revisions of "MiPNet21.07 MitoFit DatLab PT"

From Bioblast
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{{Publication
{{Publication
|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] MitoFit DatLab proficiency test. - Β 
|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] MitoFit DatLab proficiency test. -
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/index.php?title=Special:Upload&wpDestFile=MiPNet21.07_MitoFit_DatLab_proficiency_test.pdf|Bioblast pdf]] Β 
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/index.php?title=Special:Upload&wpDestFile=MiPNet21.07_MitoFit_DatLab_proficiency_test.pdf|Bioblast pdf]]
|authors=OROBOROS
|authors=OROBOROS
|year=2016-01-21
|year=2016-01-21
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# Proficiency test: Two-point O<sub>2</sub> calibration, with O<sub>2</sub> solubility factor.
# Proficiency test: Two-point O<sub>2</sub> calibration, with O<sub>2</sub> solubility factor.
# Obtain a measure of noise of instrumental O<sub>2</sub> background parameters due to variability in setting marks on 'flux'.
# Obtain a measure of noise of instrumental O<sub>2</sub> background parameters due to variability in setting marks on 'flux'.
===Files to download:===
# DatLab proficiency test instructions: [[Media:DatLabanalysisTest_instructions.pdf|Instructions]]
# Experimental data file: [[Media:file_exp1_xx.DLD|2015-09-24 P1-01.DLD]]
# Template: [[Media:Template1 xx.xls|NS 1PM 2D 3G 4c 5S 6U 7Rot 8Ama.xls]]
# Calibration and Background file: [[Media:file_bgr1_xx.DLD|2015-03-30 P1-01.DLD]]

Revision as of 11:41, 25 April 2016

Publications in the MiPMap
O2k-Protocols
MitoFit DatLab proficiency test. -

Β» Bioblast pdf

OROBOROS (2016-01-21) Mitochondr Physiol Network

Abstract: Krumschnabel G, Plattner C, Gnaiger (2016) MitoFit DatLab proficiency test. Mitochondr Physiol Network 21.07(01):1-##. Versions

The MitoFit DatLab analysis test can be considered as a first-level 'dry' MitoFit Proficiency Test for evaluation of inter-individual and inter-laboratory reproducibility and accuracy of DatLab analysis of the same data files. Proficiency will be tested for (1) setting Marks for analysis of flux; (2) O2 calibration at air saturation and zero O2 concentration; (3) analysis of instrumental O2 background.



Β» DatLab


β€’ O2k-Network Lab: AT_Innsbruck_OROBOROS


Labels: MiParea: Instruments;methods 


Organism: Human 

Preparation: Permeabilized cells 


Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property. 

HRR: Oxygraph-2k, O2k-Protocol 

MitoFitPublication 

Aims

  1. Obtain a measure of noise due to variability in setting marks on 'flux' when evaluating flux in different respiratory states.
  2. Guide the user to the DatLab-Excel template for analysis.
  3. Proficiency test: Two-point O2 calibration, with O2 solubility factor.
  4. Obtain a measure of noise of instrumental O2 background parameters due to variability in setting marks on 'flux'.