MiPNet21.07 MitoFit DatLab PT: Difference between revisions
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The MitoFit DatLab analysis test can be considered as a first-level 'dry' MitoFit Proficiency Test for evaluation of inter-individual and inter-laboratory reproducibility and accuracy of DatLab analysis of the same data files. Proficiency will be tested for (1) setting Marks for analysis of flux; (2) O2 calibration at air saturation and zero O2 concentration; (3) analysis of instrumental O2 background and copying of parameters for flux analysis. | The MitoFit DatLab analysis test can be considered as a first-level 'dry' MitoFit Proficiency Test for evaluation of inter-individual and inter-laboratory reproducibility and accuracy of DatLab analysis of the same data files. Proficiency will be tested for (1) setting Marks for analysis of flux; (2) O2 calibration at air saturation and zero O2 concentration; (3) analysis of instrumental O2 background and copying of parameters for flux analysis. | ||
===Aims=== | ===Aims=== | ||
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===Files to download:=== | ===Files to download:=== | ||
# | # DatLab proficiency test instructions: [[Media:DatLabanalysisTest_instructions.pdf|Instructions]] | ||
# Experimental data file: [[ | # Experimental data file: [[Media:file_exp1_xx.DLD|file_exp1_xx.DLD]] | ||
# Template: [[ | # Template: [[Media:Template1 xx.xls|Template1 xx.xls]] | ||
# Calibration and Background file: [[ | # Calibration and Background file: [[Media:file_bgr1_xx.DLD|file_bgr1_xx.DLD]] | ||
Revision as of 11:41, 28 January 2016
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OROBOROS (2016-01-21) Mitochondr Physiol Network
Abstract: Krumschnabel G, Plattner C, Gnaiger (2016) MitoFit DatLab proficiency test. Mitochondr Physiol Network 21.07(01):1-##.
- » Instrument: O2k, DatLab, O2k-Catalogue
- Page in prep.
• O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels: MiParea: Instruments;methods
Organism: Human
Preparation: Permeabilized cells
Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, O2k-Protocol
O2k-SOP, MitoFitPublication
Abstract:
The MitoFit DatLab analysis test can be considered as a first-level 'dry' MitoFit Proficiency Test for evaluation of inter-individual and inter-laboratory reproducibility and accuracy of DatLab analysis of the same data files. Proficiency will be tested for (1) setting Marks for analysis of flux; (2) O2 calibration at air saturation and zero O2 concentration; (3) analysis of instrumental O2 background and copying of parameters for flux analysis.
Aims
- Obtain a measure of noise due to variability in setting marks on 'flux' when evaluating flux in different respiratory states.
- Guide the user to the DatLab-Excel template for analysis.
- Proficiency test: Two-point O2 calibration, with O2 solubility factor.
- Obtain a measure of noise of instrumental O2 background parameters due to variability in setting marks on 'flux'.
- Proficiency test: Application of instrumental O2 background to analysis of flux.
Files to download:
- DatLab proficiency test instructions: Instructions
- Experimental data file: file_exp1_xx.DLD
- Template: Template1 xx.xls
- Calibration and Background file: file_bgr1_xx.DLD
