MiR05Cr: Difference between revisions
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The '''oxygen solubility factor''' of MiR05Cr is the same as for [[MiR05]] = '''0.92'''. | * For preparation of MiR06 and application in HRR, see [[MiPNet14.13 Medium-MiR06]] | ||
* The '''oxygen solubility factor''' of MiR05Cr is the same as for [[MiR05]] = '''0.92'''. | |||
'''Rationale:''' as for [[MiR05]], but less ADP is needed in permeabilized muscle fibres. This is of advantage when using the TPP electrode, because ADP has a high chemical side effect. | '''Rationale:''' as for [[MiR05]], but less ADP is needed in permeabilized muscle fibres. This is of advantage when using the TPP electrode, because ADP has a high chemical side effect. |
Revision as of 08:39, 18 July 2012
Description
Mitochondrial respiration medium, MiR05Cr, developed for oxygraph incubations of mitochondrial preparations - permeabilized muscle fibres.
MiR05Cr = MiR05 + 20 mM creatine.
Abbreviation: MiR05Cr
Reference: MiPNet14.13
MitoPedia methods:
Respirometry
MitoPedia topics:
Media for respirometry
- For preparation of MiR06 and application in HRR, see MiPNet14.13 Medium-MiR06
- The oxygen solubility factor of MiR05Cr is the same as for MiR05 = 0.92.
Rationale: as for MiR05, but less ADP is needed in permeabilized muscle fibres. This is of advantage when using the TPP electrode, because ADP has a high chemical side effect.