Difference between revisions of "Myxothiazol"
From Bioblast
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{{MitoPedia O2k and high-resolution respirometry}} | {{MitoPedia O2k and high-resolution respirometry}} | ||
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
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== Compare == | == Compare == | ||
* [[Antimycin A]] is less expensive than Myx but induces higher ROS production. | * [[Antimycin A]] is less expensive than Myx but induces higher ROS production. | ||
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|mitopedia topic=Inhibitor | |||
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Revision as of 16:23, 8 September 2020
Description
Myxothiazol, Myx, is an inhibitor of Complex III (CIII). CIII also inhibits CI (G. Lenaz). Myxothiazol binds to the Qo site of CIII (close to cytochrome bL) and inhibits the transfer of electrons from reduced QH2 to the Rieske iron sulfur protein.
Abbreviation: Myx
Reference: MiPNet09.12
Application in HRR
Myx: Myxothiazol (from Myxobacterium myxococcus fulvus, ~95%, HPLC), C25H33N3O3S2); Sigma T 5580, 1 mg, store at 4 ยฐC: FW = 487.7. Available again from Sigma with this new product number. The old product number was M 5779.
Caution: Very toxic.
Preparation of 1 mM storage and stock solution (dissolved in 99.9% ethanol):
- Weigh 1 mg of myxothiazol into a small glass vial.
- Add 2.05 mL ethanol.
- Divide into 0.2 mL (use glass vials).
- Store at -20 ยฐC.
O2k manual titrations MiPNet09.12
- Titration volume: 1 ยตL using a 10 ยตL syringe (2 mL O2k-chamber).
- Final concentration: 0.5 ยตM.
Compare
- Antimycin A is less expensive than Myx but induces higher ROS production.
MitoPedia topics: Inhibitor