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Abstract:	NextGen-O2k Summit. Virtual, 2021.
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MiPareas:	Respiration Instruments;methods mt-Biogenesis;mt-density mt-Structure;fission;fusion mt-Membrane mtDNA;mt-genetics nDNA;cell genetics Genetic knockout;overexpression Comparative MiP;environmental MiP Gender Developmental biology Exercise physiology;nutrition;life style mt-Medicine Patients Pharmacology;toxicology mt-Awareness
Pathological states:	Aging;senescence Alzheimer's Autism Cancer Cardiovascular COPD Diabetes Inherited Infectious Myopathy Neurodegenerative Obesity Parkinson's Sepsis Other
Stress conditions:	Cell death Cryopreservation Ischemia-reperfusion Permeability transition Oxidative stress;RONS Temperature Hypoxia Mitochondrial disease
Organism:	Human Pig Mouse Rat Guinea pig Bovines Horse Dog Rabbit Cat Other mammals Chicken Zebrafish Drosophila Artemia Caenorhabditis elegans Saccharomyces cerevisiae Birds Reptiles Amphibians Fishes Chelicerates Crustaceans Hexapods Myriapods Annelids Molluscs Nematodes Other invertebrates Fungi Plants Protists Eubacteria Archea
Tissues;cells:	Heart Skeletal muscle Nervous system Liver Kidney Lung;gill Islet cell;pancreas;thymus Endothelial;epithelial;mesothelial cell Blood cells Fat Genital Other cell lines CHO HEK HeLa Neuroblastoma Fibroblast HUVEC Macrophage-derived Lymphocyte Platelet Stem cells
Preparations:	Intact organism Intact organ Permeabilized cells Permeabilized tissue Homogenate Isolated mitochondria SMP Chloroplasts Enzyme Oxidase;biochemical oxidation Intact cells
Enzyme:	Adenine nucleotide translocase Complex I Complex II;succinate dehydrogenase Complex III Complex IV;cytochrome c oxidase Complex V;ATP synthase Inner mt-membrane transporter Marker enzyme Supercomplex TCA cycle and matrix dehydrogenases Uncoupling protein
Regulation; kinetics:	Aerobic glycolysis ADP ATP ATP production AMP Calcium Coupling efficiency;uncoupling Cyt c Flux control Inhibitor Ion;substrate transport mt-Membrane potential Oxygen kinetics PCr;Cr pH Phosphate Redox state Substrate Temperature Threshold;excess capacity Uncoupler Fatty acid Amino acid Q-junction effect
Coupling:	LEAK ROUTINE OXPHOS ET
Pathways:	F N S Gp DQ CIV NS Other combinations ROX
High-resolution respirometry:	Oxygraph-2k TIP2k O2k-Fluorometer pH NO TPP Ca O2k-Spectrophotometer O2k-Manual O2k-Protocol Theory NextGen-O2k
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Free text: If the abstract is longer than 500 words, please enter additional information here. {{Template:NextGen-O2k header page name}} {{MitoFit highlights page name}} <big><big>'''The revolutionary all-in-one instrument: caring about mitochondrial disease to climate change'''</big></big> [[File:NextGenO2k logo.jpg|450px|right|NextGen-O2k]] __TOC__ == General information == :::: The '''NextGen-O2k Summit''' introduces the NextGen-O2k to the scientific community with presentations from and a panel discussion with the project's Key Opinion Leaders. == Registration == :::: Register [https://attendee.gotowebinar.com/register/2704408695900902414 here] == Program == {| class="wikitable" ! Time (CET) !! Title !! Speaker |- | 16:00 || Welcome and introduction: NextGen-O2k and the Oroboros Ecosystem || [[Gnaiger Erich]] |- | 16:10 || Simultaneous measurement of coenzyme Q redox state and respiration using the NextGen-O2k || [[Komlodi Timea]] |- | 16:15 || Simultaneous measurement of mitochondrial respiration and NAD(P)H autofluorescence using the NextGen-O2k || [[Doerrier Carolina]] |- | 16:20 || '''Keynote:''' Regulation of purified Trypanosomal AOX by the redox poise of the Q-pool in rat liver mitochondria || [[Moore Anthony L|Moore Tony]] |- | 16:30 || '''Keynote:''' Bioenergetics in permeabilized muscle fibers. How far are we from achieving high-resolution respirometry, ubiquinone redox state and membrane potential readouts from same sample? || [[Garcia-Roves PM|Garcia-Roves Pablo Miguel]] |- | 16:40 || '''Keynote:''' On the consequences of genetically-determined mitochondrial defects on CoQ pool || [[Viscomi Carlo|Viscomi Carlo]] |- | 16:50 || '''Keynote:''' How substrate use and defects of respiratory Complexes influence Q-pool reduction status in isolated heart mitochondria || [[Szibor M|Szibor Marten]] |- | 17:00 || General discussion || [[Cardoso Luiza HD]], [[Donnelly Chris]] |- | 17:25 || Breather || |- | || || |- | 17:30 || '''Keynote:''' Beyond oxygen consumption rate: recording changes in ubiquinone redox state, NAD(P)H autofluorescence and membrane potential in anoxic mitochondria || [[Chinopoulos C|Chinopoulos Christos]] |- | 17:50 || '''Keynote:''' Towards defining age-related human bioenergetic decline || [[Molina Anthony JA]] |- | 18:10 || Oxia - the O<sub>2</sub> regime controller for HyperOxia and HypOxia in the O2k || [[Schmitt Sabine]] |- | 18:15 || Break || |- | || || |- | 18:25 || PhotoBiology: from photosynthesis to dark respiration || [[Gnaiger Erich]] |- | 18:30 || '''Keynote:''' The PhotoBiology module. Translating high-resolution respirometry to photosynthetic organisms: from green microalgae to land plants. || [[Vera Vives Antoni Mateu|Vera Vives Antoni Mateu]] |- | 18:40 || '''Keynote:''' Simultaneous measurements of chlorophyll fluorescence and oxygen for insights into photo-regulation of algae || [[Roach Thomas]] |- | 18:50 || Discussion and summary || |} == Speakers == {| class="wikitable" |- ! ! Participant ! Institution ! Title |- | [[File:CardosoLHD.JPG|70px|Luiza HD Cardoso]] | [[Cardoso Luiza HD]] | '''[[AT Innsbruck Oroboros]]''' - Oroboros Instruments, Innsbruck (AT) | ''t.b.c.'' |- | [[File:Christos Chinopoulos.jpg|70px|Christos Chinopoulos]] | [[Chinopoulos C|Chinopoulos Christos]] | '''[[HU Budapest Chinopoulos C]]''' - Semmelweis University, Budapest (HU) | ''Beyond oxygen consumption rate: recording changes in ubiquinone redox state, NAD(P)H autofluorescence and membrane potential in anoxic mitochondria'' |- | [[File:DoerrierC.JPG|70px|Carolina Doerrier]] | [[Doerrier Carolina]] | '''[[AT Innsbruck Oroboros]]''' - Oroboros Instruments, Innsbruck (AT) | ''Simultaneous measurement of mitochondrial respiration and NAD(P)H autofluorescence using the NextGen-O2k'' |- | [[File:Donnellyc.jpg|70px|Chris Donnelly]] | [[Donnelly Chris]] | '''[[CH Lausanne Place N]]''' - The Institute of Sport Sciences of the University of Lausanne (ISSUL), Lausanne (CH) and '''[[AT Innsbruck Oroboros]]''' - Oroboros Instruments, Innsbruck (AT) | ''t.b.c.'' |- | [[File:Garcia-RovesP.jpg|70px|Garcia-Roves Pablo]] | [[Garcia-Roves PM|Garcia-Roves Pablo Miguel]] | '''[[ES Barcelona Garcia-Roves PM]]''' - University of Barcelona and Bellvitge Biomedical Research Institute (IDIBELL), Barcelona (ES) | ''Bioenergetics in permeabilized muscle fibers. How far are we from achieving high-resolution respirometry, ubiquinone redox state and membrane potential readouts from same sample?'' |- | [[File:Erich Gnaiger.jpg|70px|Gnaiger Erich]] | [[Gnaiger Erich]] | '''[[AT Innsbruck Oroboros]]''' - Oroboros Instruments, Innsbruck (AT) | ''NextGen-O2k and the Oroboros Ecosystem'' / ''PhotoBiology: from photosythesis to dark respiration'' |- | [[File:KomlodiT.JPG|70px|Timea Komlodi]] | [[Komlodi Timea]] | '''[[AT Innsbruck Oroboros]]''' - Oroboros Instruments, Innsbruck (AT) | ''Simultaneous measurement of coenzyme Q redox state and respiration using the NextGen-O2k'' |- | [[File:Molina-Anthony-J.jpg|70px|Anthony JA Molina]] | [[Molina Anthony JA]] | '''[[US CA San Diego Molina AJA]]''' - University of California San Diego, San Diego (US) | ''Towards defining age-related human bioenergetic decline'' |- | [[File:Moore Anthony L.jpg|70px|Tony Moore]] | [[Moore Anthony L|Moore Tony]] | '''[[UK Brighton Moore AL]]''' - University of Sussex, Brighton (UK) | ''Regulation of purified Trypanosomal AOX by the redox poise of the Q-pool in rat liver mitochondria'' |- | [[File:Roach T.jpg|70px|Roach Thomas]] | [[Roach Thomas]] | University of Innsbruck, Innsbruck (AT) | ''Simultaneous measurements of chlorophyll fluorescence and oxygen for insights into photo-regulation of algae'' |- | [[File:SchmittS.jpg|70px|Schmitt Sabine]] | [[Schmitt Sabine]] | '''[[AT Innsbruck Oroboros]]''' - Oroboros Instruments, Innsbruck (AT) | ''Oxia - the O<sub>2</sub> regime controller for HyperOxia and HypOxia in the O2k'' |- | [[File:Profile-icon-9.png|70px|Participant]] | [[Szibor M|Szibor Marten]] | '''[[DE Jena Szibor M]]''' - Jena University Hospital, Jena (DE) | ''How substrate use and defects of respiratory Complexes influence Q-pool reduction status in isolated heart mitochondria'' |- | [[File:Vera Vives AM.jpeg|70px|Vera Vives Antoni Mateu]] | [[Vera Vives Antoni Mateu|Vera Vives Antoni Mateu]] | '''IT Padova Morosinotto T''' - Università degli Studi di Padova, Padova (IT) | ''The PhotoBiology module. Translating high resolution respirometry to photosynthetic organisms: from green microalgae to land plants'' |- | [[File:Profile-icon-9.png|70px|Participant]] | [[Viscomi Carlo|Viscomi Carlo]] | '''[[IT Padova Viscomi C]]''' - University of Padova, Padova (IT) | ''On the consequences of genetically-determined mitochondrial defects on CoQ pool'' |- |} == Contact == :::: [mailto:instruments@oroboros.at| instruments@oroboros.at] :::: '''Oroboros Instruments''' :::: High-Resolution Respirometry :::: Schoepfstrasse 18 :::: A-6020 Innsbruck, Austria :::: Tel: +43 512 566796 :::: Fax: +43 512 566796 20 :::: '''Mitochondria and Cell Research''' == Support == [[File:Template NextGen-O2k.jpg|right|400px|link=NextGen-O2k]] :::: Supported by project NextGen-O2k which has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 859770.

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