Difference between revisions of "O2k-Publications: Fibroblast"
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'''NIH3T3''' mouse embryonic fibroblast cells come from a cell line isolated and initiated in 1962 at the New York University School of Medicine Department of Pathology. 3T3 refers to the cell transfer and inoculation protocol for the line, and means ''3-day transfer, inoculum 3 x 10<sup>5</sup> cells''. Using this protocol, the immortal cell line begins to thrive and stabilize in cell culture after about 20-30 generations of ''in vitro'' growth. George Todaro and Howard Green, the scientists who first cultured this cell line, obtained the cells from desegregated NIH Swiss mouse embryo fibroblasts. The cell line has since become a standard fibroblast cell line. | |||
[http://nih3t3.com/nih3t3_cell_culture.html Cell Culture Information] | |||
Revision as of 11:51, 13 February 2013
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NIH3T3 mouse embryonic fibroblast cells come from a cell line isolated and initiated in 1962 at the New York University School of Medicine Department of Pathology. 3T3 refers to the cell transfer and inoculation protocol for the line, and means 3-day transfer, inoculum 3 x 105 cells. Using this protocol, the immortal cell line begins to thrive and stabilize in cell culture after about 20-30 generations of in vitro growth. George Todaro and Howard Green, the scientists who first cultured this cell line, obtained the cells from desegregated NIH Swiss mouse embryo fibroblasts. The cell line has since become a standard fibroblast cell line.
