Difference between revisions of "OctPM pathway control state"
From Bioblast
| Line 10: | Line 10: | ||
|info=[[MiPNet21.06 SUIT reference protocol]] | |info=[[MiPNet21.06 SUIT reference protocol]] | ||
}} | }} | ||
{{MitoPedia concepts}} | {{MitoPedia concepts | ||
|mitopedia concept=Respiratory state, SUIT state | |||
}} | |||
{{MitoPedia methods}} | {{MitoPedia methods}} | ||
{{MitoPedia O2k and high-resolution respirometry | {{MitoPedia O2k and high-resolution respirometry}} | ||
}} | |||
{{MitoPedia topics}} | {{MitoPedia topics}} | ||
== PMOct(L) == | == PMOct(L) == | ||
Revision as of 16:20, 13 May 2016
- high-resolution terminology - matching measurements at high-resolution
OctPM pathway control state
Description
PMOct: Pyruvate & Malate & Octanoylcarnitine.
MitoPathway control: CI&FAO
SUIT protocol: SUIT-RP1, SUIT-RP1
This substrate combination supports CI-linked flux which is typically higher than FAO capacity. In SUIT-RP1, PMOct is induced after PM(E), to evaluate any additive effect of adding Oct. In SUIT-RP2, FAO OXPHOS capacity is measured first, testing for the effect of increasing malate concentration (compare Malate alone, and pyruvate is added to compare FAO as the background state with CI&FAO as the reference state.
Abbreviation: PMOct
Reference: MiPNet21.06 SUIT reference protocol
MitoPedia concepts:
Respiratory state,
SUIT state
PMOct(L)
PMOct(P)
- SUIT-RP2_4P: 1D 2Oct 3Mtit 3c (3NADH) 4P 5G 6S 7U 8Gp 9Rot 10Ama 11Tm 12Azd
PMOct(E)
- SUIT-RP1_4Oct: 1PM 2D 2c (2NADH) 3U 4Oct 5G 6S 7Rot 8Gp 9Ama 10Tm 11Azd
