Difference between revisions of "OctPM pathway control state"
From Bioblast
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'''MitoPathway control:''' CI<small>&</small>FAO | '''MitoPathway control:''' CI<small>&</small>FAO | ||
'''SUIT protocol:''' [[SUIT | '''SUIT protocol:''' [[SUIT RP1]], [[SUIT RP1]] | ||
This substrate combination supports CI-linked flux which is typically higher than FAO capacity. In SUIT-RP1, PMOct is induced after PM(E), to evaluate any additive effect of adding Oct. In SUIT-RP2, FAO OXPHOS capacity is measured first, testing for the effect of increasing malate concentration (compare [[Malate alone]], and pyruvate is added to compare FAO as the background state with CI&FAO as the reference state. | This substrate combination supports CI-linked flux which is typically higher than FAO capacity. In SUIT-RP1, PMOct is induced after PM(E), to evaluate any additive effect of adding Oct. In SUIT-RP2, FAO OXPHOS capacity is measured first, testing for the effect of increasing malate concentration (compare [[Malate alone]], and pyruvate is added to compare FAO as the background state with CI&FAO as the reference state. | ||
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== PMOct(P) == | == PMOct(P) == | ||
# [[SUIT | # [[SUIT RP2]]_'''4P''': 1D 2Oct 3Mtit 3c (3NADH) '''<u>4P</u>''' 5G 6S 7U 8Gp 9Rot 10Ama 11Tm 12Azd''' | ||
== PMOct(E) == | == PMOct(E) == | ||
# [[SUIT | # [[SUIT RP1]]_'''4Oct''': 1PM 2D 2c (2NADH) 3U '''<u>4Oct</u>''' 5G 6S 7Rot 8Gp 9Ama 10Tm 11Azd | ||
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Revision as of 12:15, 20 July 2016
- high-resolution terminology - matching measurements at high-resolution
OctPM pathway control state
Description
PMOct: Pyruvate & Malate & Octanoylcarnitine.
MitoPathway control: CI&FAO
SUIT protocol: SUIT RP1, SUIT RP1
This substrate combination supports CI-linked flux which is typically higher than FAO capacity. In SUIT-RP1, PMOct is induced after PM(E), to evaluate any additive effect of adding Oct. In SUIT-RP2, FAO OXPHOS capacity is measured first, testing for the effect of increasing malate concentration (compare Malate alone, and pyruvate is added to compare FAO as the background state with CI&FAO as the reference state.
Abbreviation: PMOct
Reference: MiPNet21.06 SUIT reference protocol
MitoPedia concepts:
Respiratory state,
SUIT state
PMOct(L)
PMOct(P)
- SUIT RP2_4P: 1D 2Oct 3Mtit 3c (3NADH) 4P 5G 6S 7U 8Gp 9Rot 10Ama 11Tm 12Azd
PMOct(E)
- SUIT RP1_4Oct: 1PM 2D 2c (2NADH) 3U 4Oct 5G 6S 7Rot 8Gp 9Ama 10Tm 11Azd
