Difference between revisions of "Oligomycin"
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Revision as of 16:50, 9 March 2011
- high-resolution terminology - matching measurements at high-resolution
Oligomycin
Description
Oligomycin is an inhibitor of ATP synthase.
Abbreviation: Omy
Reference: MiPNet09.12
MitoPedia topics:
Inhibitor
Application in HRR
Omy: Oligomycin (Oligomycin from Streptomyces diastatochromogenes, mixture of oligomycins A, B, and C); Sigma O 4876, 5 mg, store at -20 °C: FW = 800
Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
Preparation of 4 mg/ml (5 mM) stock solution (dissolved in 99.9% Ethanol):
- 1) weigh 4 mg of Oligomycin into a 2 ml glass vial and dissolve in 1 ml Ethanol
- 2) divide into 0.2 ml portions OR store in 2 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution
- 3) store in glass vials (recommendation) at -20 °C
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 1 µl using a 10 µl syringe
- Final conc. in 2 ml O2k-chamber: 2 µg/ml (2.5 µM)
