Difference between revisions of "Oligomycin"
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|enzymes=Complex V; ATP Synthase | |||
|kinetics=Inhibitor; Uncoupler | |kinetics=Inhibitor; Uncoupler | ||
| | |instruments=Chemicals; Media | ||
|type=Inhibitor PS | |||
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== Application in [[HRR]] == | |||
'''Omy: Oligomycin''' (Oligomycin from ''Streptomyces diastatochromogenes'', mixture of oligomycins A, B, and C); Sigma O 4876, 5 mg, store at -20 °C: FW = 800 | |||
<span style="color:#8B008B"> '''Caution:''' Very toxic. Chemicals stored in the fridge or freezer should be adjusted to room temperature before opening.</span> | |||
'''Preparation of 4mg/ml stock solution''' (dissolved in 99.9% Ethanol): | |||
::1) weigh 4 mg of Oligomycin directly into a 2 ml glass vial | |||
::2) add 1 ml Ethanol | |||
::3) divide into 0.2 ml portions OR store in 2 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution | |||
::4) store in glass vials (recommendation) at -20 °C | |||
'''Oxygraph-2k Manual Titrations:''' [[MiPNet09.12]] | |||
::* Titration volume: 1 µl using a 10 µl syringe | |||
::* Final conc. in 2 ml O2k-chamber: 2 µg/ml | |||
Revision as of 13:58, 22 November 2010
- high-resolution terminology - matching measurements at high-resolution
Oligomycin
Description
Oligomycin is an inhibitor of ATP synthase.
Abbreviation: Omy
Reference: MiPNet09.12
Labels:
Enzyme: Complex V; ATP Synthase"Complex V; ATP Synthase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property.
HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
Application in HRR
Omy: Oligomycin (Oligomycin from Streptomyces diastatochromogenes, mixture of oligomycins A, B, and C); Sigma O 4876, 5 mg, store at -20 °C: FW = 800
Caution: Very toxic. Chemicals stored in the fridge or freezer should be adjusted to room temperature before opening.
Preparation of 4mg/ml stock solution (dissolved in 99.9% Ethanol):
- 1) weigh 4 mg of Oligomycin directly into a 2 ml glass vial
- 2) add 1 ml Ethanol
- 3) divide into 0.2 ml portions OR store in 2 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution
- 4) store in glass vials (recommendation) at -20 °C
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 1 µl using a 10 µl syringe
- Final conc. in 2 ml O2k-chamber: 2 µg/ml
