Difference between revisions of "Ouabain"
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:::'''Oua: Ouabain octahydrate''' (≥95% (HPLC), powder; C<sub>29</sub>H<sub>44</sub>O<sub>12</sub><sub>*</sub>8H<sub>2</sub>0); Sigma O 3125, store at R.T.; FW = 728.77. | :::'''Oua: Ouabain octahydrate''' (≥95% (HPLC), powder; C<sub>29</sub>H<sub>44</sub>O<sub>12</sub><sub>*</sub>8H<sub>2</sub>0); Sigma O 3125, store at R.T.; FW = 728.77. | ||
<span style="color:#8B008B"> '''Caution:''' Light sensitive and toxic!</span> | ::::<span style="color:#8B008B"> '''Caution:''' Light sensitive and toxic!</span> | ||
::::'''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O): | ::::'''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O): | ||
Revision as of 10:31, 11 September 2020
- high-resolution terminology - matching measurements at high-resolution
Ouabain
Description
Ouabain (synonym: G-strophantin octahydrate) is a poisonous cardiac glycoside. The classical mechanism of action of ouabain involves its binding to and inhibition of the plasma membrane Na+/K+-ATPase (sodium pump) especially at the higher concentrations. Low (nanomolar and subnanomolar) concentrations of ouabain stimulate the Na-K-ATPase.
Abbreviation: Oua
Reference: MiPNet03.02
Application in HRR
- Oua: Ouabain octahydrate (≥95% (HPLC), powder; C29H44O12*8H20); Sigma O 3125, store at R.T.; FW = 728.77.
- Caution: Light sensitive and toxic!
- Preparation of 10 mM stock solution (dissolved in H2O):
- Weigh 7.3 mg ouabain.
- Dissolve in 1 mL H2O.
- Divide into 0.2 mL portions or store in 2 mL glass vial and use subsamples during experiments to avoid contamination of the storage solution.
- Store in glass vials (recommendation) at -20 °C.
MitoPedia topics:
Inhibitor
